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PMID: 1332044 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Basonuclin: a keratinocyte protein with multiple paired zinc fingers.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 21 ·1992-11-01 ·Pages 10311-5

Tseng H, Green H

Abstract

A cDNA clone has been prepared from mRNA of cultured human keratinocytes. The sequence of the cDNA reveals that in the C-terminal two-thirds of the corresponding protein (basonuclin), there are three separated pairs of adjacent zinc fingers. The amino acid sequence of each pair is homologous to that of the single pair of zinc fingers of the Drosophila transcription factor encoded by disco. Near the C-terminal end of basonuclin and on the surface of a putative alpha-helix, there is a stripe of serine residues similar to that of the transcription factor PRDII-BF1. Basonuclin possesses a sequence of six amino acids quite similar to one present in the myogenic family of proteins, including Myf5; this sequence is located in the omega loop of the myogenic proteins but within a zinc finger of the keratinocyte protein. As basonuclin is present mainly in the nuclei of the basal cell layer, its regulatory function is likely to be exerted prior to the process of terminal differentiation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA/genetics,isolation & purification DNA-Binding Proteins Gene Library Humans Infant, Newborn Keratinocytes/physiology Male Molecular Sequence Data Muscle Proteins/genetics MyoD Protein Phosphoproteins Protein Conformation Proteins/genetics RNA, Messenger/genetics,isolation & purification Restriction Mapping Sequence Homology, Amino Acid Skin Physiological Phenomena Transcription Factors Zinc Fingers/genetics
Chemicals
DNA-Binding Proteins Muscle Proteins MyoD Protein Phosphoproteins Proteins RNA, Messenger Transcription Factors BNC1 protein, human DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tseng H
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02115.
Green H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.21.10311
Published
1992-11-01
Pages
10311-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50328
Subset
IM
Databases
GENBANK
L03427
External Links
PubMed source DOI: 10.1073/pnas.89.21.10311 PMC Full Text View journal details
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