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PMID: 1846092 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The gene teashirt is required for the development of Drosophila embryonic trunk segments and encodes a protein with widely spaced zinc finger motifs.

Cell ·Vol. 64 ·No. 1 ·1991-01-11 ·Pages 63-79

Fasano L, Röder L, Coré N, Alexandre E, Vola C, Jacq B, Kerridge S

Abstract

We have discovered a reporter gene insertion that is expressed in the trunk region of Drosophila embryos. Genetic and molecular details of a new regulatory gene neighboring the reporter gene insertion, which we call teashirt (tsh), are described. In situ hybridization of a tsh probe to embryos shows that this gene is expressed in a way similar to the reporter gene. Mutations of tsh show that the gene is required for normal development of the ventral trunk region of embryos, which correlates with the spatial expression of the gene in the anteroposterior axis but not in the dorsoventral axis. Sequencing of a tsh cDNA shows that the putative protein possesses three distantly spaced CX2CX12HX5H zinc finger motifs.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Chromosome Deletion Chromosomes/ultrastructure Cloning, Molecular DNA Transposable Elements Drosophila/embryology,genetics Drosophila Proteins Embryo, Nonmammalian/physiology Female Genes, Regulator Genetic Vectors Insect Hormones Male Molecular Sequence Data Mutagenesis Proteins/genetics RNA, Antisense/genetics Recombinant Fusion Proteins/metabolism Repressor Proteins Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors Zinc Fingers/genetics beta-Galactosidase/genetics,metabolism

Chemicals

DNA Transposable Elements Drosophila Proteins Insect Hormones Proteins RNA, Antisense Recombinant Fusion Proteins Repressor Proteins Transcription Factors tsh protein, Drosophila beta-Galactosidase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Fasano L

CNRS, Centre Universitaire de Marseille-Luminy, France.

Röder L

Coré N

Alexandre E

Vola C

Jacq B

Kerridge S

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(91)90209-h

Published

1991-01-11

Pages

63-79

Language

English

Region

United States

NLM ID

0413066

Subset

Databases

GENBANK

M57496, M59201, M63698, M63700, M63702, M63718, S63145, S63146, S63147, S63148

External Links

PubMed source DOI: 10.1016/0092-8674(91)90209-h View journal details

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The gene teashirt is required for the development of Drosophila embryonic trunk segments and encodes a protein with widely spaced zinc finger motifs.

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