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PMID: 1323219 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stromal expression of 72 kda type IV collagenase (MMP-2) and TIMP-2 mRNAs in colorectal neoplasia.

The American journal of pathology ·Vol. 141 ·No. 2 ·1992-08-00 ·Pages 389-96

Poulsom R, Pignatelli M, Stetler-Stevenson WG, Liotta LA, Wright PA, Jeffery RE, Longcroft JM, Rogers L, Stamp GW

Abstract

We undertook an in situ hybridization study to localize the mRNAs for the 72 kda type IV collagenase (MMP-2) and its specific inhibitor (TIMP-2) in 12 colorectal carcinomas, 3 adenomas, and 4 uninvolved resection margins to see how their distributions correlated with that of the reported distribution of MMP-2 protein. Labeling for MMP-2 and TIMP-2 mRNAs was detectable in 10 of 12 carcinomas and in 2 of 3 adenomas. Unexpectedly, we found much stronger signals for MMP-2 and TIMP-2 mRNAs within the mesenchymal cells in the desmoplastic stroma, of endothelial and/or (myo)fibroblastic nature, rather than in tumor epithelial cells in which localization of MMP-2 was anticipated. Our data indicate that stromal cells may have the ability to synthesize a metalloproteinase that degrades basement membrane, and may together with the neoplastic epithelial cells participate actively in the tissue remodeling and disruption of the basement membrane integrity which is characteristic of invasive tumors.

MeSH Terms
Actins/genetics Adenoma/genetics,metabolism Antibodies, Monoclonal/genetics Carcinoma/genetics,metabolism Colorectal Neoplasms/genetics,metabolism Glycoproteins/genetics Humans Microbial Collagenase/chemistry,genetics Molecular Weight RNA, Messenger/metabolism Tissue Inhibitor of Metalloproteinases
Chemicals
Actins Antibodies, Monoclonal Glycoproteins RNA, Messenger Tissue Inhibitor of Metalloproteinases Microbial Collagenase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Poulsom R
ICRF/RCS Histopathology Unit, London, United Kingdom.
Pignatelli M
Stetler-Stevenson W G
Liotta L A
Wright P A
Jeffery R E
Longcroft J M
Rogers L
Stamp G W
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1992-08-00
Pages
389-96
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886613
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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