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PMID: 1322370 Published · ppublish English Journal Article

Pasteurella haemolytica leukotoxin enhances production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by bovine polymorphonuclear leukocytes.

Infection and immunity ·Vol. 60 ·No. 8 ·1992-08-00 ·Pages 3238-43

Henricks PA, Binkhorst GJ, Drijver AA, Nijkamp FP

Abstract

The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic acid. The leukotoxin preparations dose dependently enhanced the release of the 5-lipoxygenase products 5-HETE and LTB4 in arachidonic acid-stimulated PMNs, whereas the release of 12- and 15-HETE was not affected. The enhanced release of LTB4 and 5-HETE was not due to a decreased cellular retention of the 5-lipoxygenase products. In addition, leukotoxin preparations by themselves were also able to induce LTB4 and 5-HETE production in the absence of exogenous arachidonic acid. Generation of 5-lipoxygenase products by PMNs stimulated by leukotoxin may represent an important cellular event that occurs during infections with P. haemolytica.

MeSH Terms
Animals Bacterial Toxins/pharmacology Cattle Cells, Cultured Exotoxins/pharmacology Humans Hydroxyeicosatetraenoic Acids/biosynthesis Leukotriene B4/biosynthesis Mannheimia haemolytica/metabolism Neutrophils/metabolism Species Specificity
Chemicals
Bacterial Toxins Exotoxins Hydroxyeicosatetraenoic Acids leukotoxin Leukotriene B4 5-hydroxy-6,8,11,14-eicosatetraenoic acid 15-hydroxy-5,8,11,13-eicosatetraenoic acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Henricks P A
Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Binkhorst G J
Drijver A A
Nijkamp F P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.8.3238-3243.1992
Published
1992-08-00
Pages
3238-43
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257307
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.60.8.3238-3243.1992 PMC Full Text View journal details
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