Effect of thiol-activated toxins (streptolysin O, alveolysin, and theta toxin) on the generation of leukotrienes and leukotriene-inducing and -metabolizing enzymes from human polymorphonuclear granulocytes.

Infection and immunity ·Vol. 50 ·No. 3 ·1985-12-00 ·Pages 844-51

Bremm KD, König W, Pfeiffer P, Rauschen I, Theobald K, Thelestam M, Alouf JE

Abstract

The generation of leukotrienes (LTC4, LTD4, LTE4, and LTB4; 12-epi-LTB4 isomer) from human granulocytes by thiol-activated toxins (streptolysin O, alveolysin from Bacillus alvei, and theta toxin from Clostridium perfringens) is described. The release occurs under noncytolytic conditions. Although LTB4 is the major component after calcium ionophore stimulation, more LTC4 as compared with LTB4 is released with the toxins. The 5-lipoxygenase pathway of toxin-mediated activation can effectively be inhibited by caffeic acid, a lipoxygenase inhibitor. The toxins also induce the release of leukotriene-metabolizing enzymes such as gamma-glutamyltranspeptidase, which transfers LTC4 into LTD4, and dipeptidase, which metabolizes LTD4, into LTE4. Dipeptidase activity is more pronounced than the gamma-glutamyltranspeptidase activity but still does not reach the levels obtained when cells were triggered with opsonized zymosan.

MeSH Terms

Arachidonate Lipoxygenases Bacterial Proteins Bacterial Toxins/pharmacology Hemolysin Proteins/pharmacology Humans In Vitro Techniques Kinetics Leukotriene B4/biosynthesis Lipoxygenase/analysis Neutrophils/drug effects,metabolism Organic Chemicals SRS-A/biosynthesis Streptolysins/pharmacology gamma-Glutamyltransferase/analysis

Chemicals

Bacterial Proteins Bacterial Toxins Hemolysin Proteins Organic Chemicals SRS-A Streptolysins streptolysin O Leukotriene B4 Clostridium perfringens theta-toxin alveolysin Arachidonate Lipoxygenases Lipoxygenase gamma-Glutamyltransferase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Bremm K D

König W

Pfeiffer P

Rauschen I

Theobald K

Thelestam M

Alouf J E

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26 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.50.3.844-851.1985

Published

1985-12-00

Pages

844-51

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC261157

Subset

External Links

PubMed source DOI: 10.1128/iai.50.3.844-851.1985 PMC Full Text View journal details

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Effect of thiol-activated toxins (streptolysin O, alveolysin, and theta toxin) on the generation of leukotrienes and leukotriene-inducing and -metabolizing enzymes from human polymorphonuclear granulocytes.

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