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PMID: 1320255 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of an internal cis-element essential for the human L1 transcription and a nuclear factor(s) binding to the element.

Nucleic acids research ·Vol. 20 ·No. 12 ·1992-06-25 ·Pages 3139-45

Minakami R, Kurose K, Etoh K, Furuhata Y, Hattori M, Sakaki Y

Abstract

L1 (LINE-1) is a long interspersed repetitive sequence derived from a retrotransposon. Transfection studies using the CAT gene as a reporter demonstrated that the first 155bp in the human L1 sequence contains an element(s) responsible for the promoter activity in HeLa cells. The transcription was shown to initiate at the first nucleotide of the L1 sequence in the transgene. Three prominent nuclear protein binding sites were found in the 5' region of the L1 sequence by DNaseI footprint analysis. One of the binding sites, designated as site A located at +3 to +26, was shown to be essential for the L1 transcription because the mutation at the site A caused almost complete loss of the promoter activity. A sequence AAGATGGCC at +11 to +19 in the site A was defined as a target core element for the protein binding. The site A-binding protein (designated TFL1-A) was found in various types of cells including an embryonic teratocarcinoma cell line. These results indicate that an internal short element located at the very 5' terminal of L1 sequence and the nuclear factor binding to the element play a crucial role in the transcription of human L1.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites/genetics Chloramphenicol O-Acetyltransferase/genetics,metabolism DNA Transposable Elements/genetics DNA-Binding Proteins/metabolism HeLa Cells Humans Luciferases/genetics,metabolism Molecular Sequence Data Plasmids/genetics Promoter Regions, Genetic/genetics Recombinant Fusion Proteins/genetics,metabolism Regulatory Sequences, Nucleic Acid/genetics Repetitive Sequences, Nucleic Acid/genetics Transcription, Genetic/genetics Transfection/genetics
Chemicals
DNA Transposable Elements DNA-Binding Proteins Recombinant Fusion Proteins Luciferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Minakami R
Research Laboratory for Genetic Information, Kyushu University, Fukuoka, Japan.
Kurose K
Etoh K
Furuhata Y
Hattori M
Sakaki Y
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.12.3139
Published
1992-06-25
Pages
3139-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312450
Subset
IM
Databases
GENBANK
X52235
External Links
PubMed source DOI: 10.1093/nar/20.12.3139 PMC Full Text View journal details
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