Home LiteratureArticle Details
PMID: 1317075 Published · ppublish English Journal Article

The complete nucleotide sequence of a variant of Coxsackievirus A24, an agent causing acute hemorrhagic conjunctivitis.

Virus genes ·Vol. 6 ·No. 2 ·1992-04-00 ·Pages 149-58

Supanaranond K, Takeda N, Yamazaki S

Abstract

The complete nucleotide sequence was determined for the cDNAs that represent the RNA genome of the standard strain of a variant of coxsackievirus A24, the EH24/70, one of the agents causing acute hemorrhagic conjunctivitis. The genome is 7461 nucleotide long and is polyadenylated at the 3'-end terminus. Following a 750-nucleotide 5'-noncoding region, there was a long open reading frame of 6642 nucleotides, which serve to encode a viral polyprotein consisting of 2214 amino acids. Comparison of the deduced amino acid sequence of the polyprotein with those of known enteroviruses allowed us to predict the possible cleavage sites. The overall structure and the organization of the RNA genome is typical for an enterovirus. Based on the similarity of the nucleotide sequence of the 5' and 3' noncoding regions, together with the amino-acid sequence of the encoded proteins, EH24/70 appeared to be closely related to polioviruses and coxsackievirus A21.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular Conjunctivitis, Acute Hemorrhagic/microbiology Coxsackievirus Infections/microbiology Enterovirus/genetics,growth & development HeLa Cells Humans Molecular Sequence Data Picornaviridae/genetics RNA, Viral/isolation & purification Sequence Homology, Nucleic Acid Viral Proteins
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Supanaranond K
Central Virus Diagnostic Laboratory, National Institute of Health, Tokyo, Japan.
Takeda N
Yamazaki S
References (34)
34 references, click to expand
  1. Enterovirus etiology of the 1970 Singapore epidemic of acute conjunctivitis.
    Intervirology. 1974;4(2):119-27 PMID: 4217326
  2. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.
    J Mol Biol. 1984 Apr 25;174(4):561-85 PMID: 6202874
  3. Comparative sequence analysis of the 5' noncoding region of the enteroviruses and rhinoviruses.
    Virology. 1988 Jul;165(1):42-50 PMID: 2838971
  4. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  5. Evolutionary study on the Coxsackievirus A 24 variant causing acute hemorrhagic conjunctivitis by oligonucleotide mapping analysis of RNA genome.
    Arch Virol. 1990;114(1-2):37-51 PMID: 2171464
  6. A recent epidemic of Coxsackie virus type A24 acute haemorrhagic conjunctivitis in Singapore.
    Br J Ophthalmol. 1986 Nov;70(11):869-73 PMID: 3024697
  7. An epidemic of picornavirus conjunctivitis in Singapore.
    Southeast Asian J Trop Med Public Health. 1972 Sep;3(3):303-9 PMID: 4631506
  8. An epidemic of acute hemorrhagic conjunctivitis due to coxsackievirus A24.
    J Infect Dis. 1982 Jul;146(1):16-9 PMID: 6282991
  9. The complete nucleotide sequence of enterovirus type 70: relationships with other members of the picornaviridae.
    J Gen Virol. 1990 Oct;71 ( Pt 10):2291-9 PMID: 2172447
  10. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  11. Complete nucleotide sequence of the genome of coxsackievirus B1.
    Virology. 1987 Jan;156(1):64-73 PMID: 3027969
  12. Acute hemorrhagic conjunctivitis caused by coxsackie A24 variant--Puerto Rico.
    MMWR Morb Mortal Wkly Rep. 1988 Mar 4;37(8):123-4, 129 PMID: 2828909
  13. Acute hemorrhagic conjunctivitis caused by coxsackievirus A24--Caribbean.
    MMWR Morb Mortal Wkly Rep. 1987 May 1;36(16):245-6, 251 PMID: 3104763
  14. Three-dimensional structure of poliovirus at 2.9 A resolution.
    Science. 1985 Sep 27;229(4720):1358-65 PMID: 2994218
  15. Systematic nomenclature of picornavirus proteins.
    J Virol. 1984 Jun;50(3):957-9 PMID: 6726891
  16. The complete nucleotide sequence of coxsackievirus A21.
    J Gen Virol. 1989 Nov;70 ( Pt 11):2943-52 PMID: 2584950
  17. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  18. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793-7 PMID: 6310545
  19. A study of the evolution of coxsackievirus A24 variant in Ghana by viral RNA fingerprinting analysis.
    Res Virol. 1991 Jan-Feb;142(1):57-65 PMID: 2052752
  20. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  21. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  22. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  23. The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.
    Nucleic Acids Res. 1983 Aug 25;11(16):5629-43 PMID: 6310508
  24. Acute haemorrhagic conjunctivitis in Hong Kong 1971-1975.
    Southeast Asian J Trop Med Public Health. 1977 Mar;8(1):1-6 PMID: 196351
  25. Analyses of the mRNA transcription processes of Punta Toro phlebovirus (Bunyaviridae).
    Virology. 1985 Dec;147(2):317-25 PMID: 2416115
  26. Evolution of enterovirus 70 in nature: all isolates were recently derived from a common ancestor.
    Arch Virol. 1986;89(1-4):1-14 PMID: 3013125
  27. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  28. The first epidemic of acute hemorrhagic conjunctivitis due to a coxsackievirus A24 variant in Okinawa, Japan, in 1985-1986.
    Jpn J Med Sci Biol. 1988 Aug;41(4):159-74 PMID: 2855085
  29. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.
    Nature. 1988 Jul 28;334(6180):320-5 PMID: 2839775
  30. Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing.
    J Virol. 1986 Feb;57(2):515-25 PMID: 3003384
  31. Etiologic studies on acute hemorrhagic conjunctivitis in Thailand 1971-1975.
    J Med Assoc Thai. 1978 Apr;61(4):195-201 PMID: 650111
  32. Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1539-43 PMID: 6324200
  33. Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine.
    Nucleic Acids Res. 1984 Oct 25;12(20):7787-92 PMID: 6093054
  34. The nucleotide sequence of 3C proteinase region of the coxsackievirus A24 variant: comparison of the isolates in Taiwan in 1985-1988.
    Virus Genes. 1991 Apr;5(2):121-31 PMID: 1647565
Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF01703064
Published
1992-04-00
Pages
149-58
Language
English
Region
United States
NLM ID
8803967
Subset
IM
Databases
GENBANK
D90457
External Links
PubMed source DOI: 10.1007/BF01703064 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2