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PMID: 1312717 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Multiple retinoid-responsive receptors in a single cell: families of retinoid "X" receptors and retinoic acid receptors in the Xenopus egg.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 6 ·1992-03-15 ·Pages 2321-5

Blumberg B, Mangelsdorf DJ, Dyck JA, Bittner DA, Evans RM, De Robertis EM

Abstract

In a search for nuclear hormone receptors expressed in early development we found that Xenopus laevis eggs contain mRNAs from two retinoic acid receptor genes (xRAR alpha and xRAR gamma) and two retinoid "X" receptor genes (xRXR alpha and xRXR gamma). We also show that RXRs are members of a family of at least three genes, thus expanding the number of genes encoding retinoic acid-responsive transcription factors to six. With the exception of xRXR gamma, these maternal mRNAs are degraded before gastrulation. The RXRs isolated are differentially activated by retinoic acid and by 3,4-didehydroretinoic acid. Considered together, these four receptors provide a molecular basis for the pleiotropic effects of retinoic acid on early development, and their pattern of expression suggests a role for retinoic acid at the earliest stages of embryonic determination.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics,isolation & purification,metabolism Embryo, Nonmammalian/physiology Female Gene Library Humans Infant, Newborn Kinetics Molecular Sequence Data Oligodeoxyribonucleotides Ovum/drug effects,physiology RNA, Messenger/genetics,metabolism Receptors, Cell Surface/genetics,isolation & purification,metabolism Receptors, Retinoic Acid Retinoid X Receptors Sequence Homology, Nucleic Acid Transcription Factors Transfection Tretinoin/metabolism,pharmacology Xenopus laevis
Chemicals
Carrier Proteins Oligodeoxyribonucleotides RNA, Messenger Receptors, Cell Surface Receptors, Retinoic Acid Retinoid X Receptors Transcription Factors Tretinoin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Blumberg B
Department of Biological Chemistry, University of California, Los Angeles Medical School 90024-1737.
Mangelsdorf D J
Dyck J A
Bittner D A
Evans R M
De Robertis E M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.6.2321
Published
1992-03-15
Pages
2321-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48649
Subset
IM
Grants
NIGMS NIH HHS · GM-07185 · United States
NICHD NIH HHS · HD-07273 · United States
NICHD NIH HHS · HD-27700 · United States
Databases
GENBANK
UNKNOWN
External Links
PubMed source DOI: 10.1073/pnas.89.6.2321 PMC Full Text View journal details
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