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PMID: 1310776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simian virus 40 large T antigen stably complexes with a 185-kilodalton host protein.

Journal of virology ·Vol. 66 ·No. 3 ·1992-03-00 ·Pages 1752-60

Kohrman DC, Imperiale MJ

Abstract

Stable interactions between simian virus 40 large T antigen and host proteins are believed to play a major role in the ability of the viral protein to transform cells in culture and induce tumors in vivo. Two of these host proteins, the retinoblastoma susceptibility protein (pRB) and p53, are products of tumor suppressor genes, suggesting that T antigen exerts at least a portion of its transforming activity by complexing with and inactivating the function of these proteins. While analyzing T antigen-host protein complexes in mouse cells, we noted a protein of 185 kDa (p185) which specifically coimmunoprecipitates with T antigen. Coimmunoprecipitation results from the formation of stable complexes between T antigen and p185. Complex formation is independent of the interactions of T antigen with pRB, p120, and p53. Furthermore, analysis of T-antigen mutants suggests that T antigen-p185 complex formation may be important in transformation by simian virus 40.

MeSH Terms
3T3 Cells Animals Antigens, Polyomavirus Transforming/chemistry Binding Sites Cell Transformation, Viral DNA Polymerase II/metabolism In Vitro Techniques Macromolecular Substances Mice Molecular Weight Precipitin Tests Protein Binding Simian virus 40/physiology Transfection
Chemicals
Antigens, Polyomavirus Transforming Macromolecular Substances DNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kohrman D C
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Imperiale M J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.3.1752-1760.1992
Published
1992-03-00
Pages
1752-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240927
Subset
IM
Grants
NCI NIH HHS · CA19816 · United States
NIGMS NIH HHS · GM07544 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.3.1752-1760.1992 PMC Full Text View journal details
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