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PMID: 1310752 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regions of human papillomavirus type 16 E7 oncoprotein required for immortalization of human keratinocytes.

Journal of virology ·Vol. 66 ·No. 3 ·1992-03-00 ·Pages 1329-35

Jewers RJ, Hildebrandt P, Ludlow JW, Kell B, McCance DJ

Abstract

Binding of the retinoblastoma gene product (pRB) by viral oncoproteins, including the E7 of human papillomavirus type 16 (HPV 16), is thought to be important in transformation of cells. One of the steps in transformation is the immortalization process. Here we show that mutations in E7 within the full-length genome which inhibit binding of pRB do not abrogate the ability of the HPV 16 DNA to immortalize primary human epithelial (keratinocyte) cells. A mutation in one of the cysteines of a Cys-X-X-Cys motif which is contained in the carboxy half of the E7 and is part of a zinc finger arrangement completely eliminates the ability of HPV 16 DNA to immortalize cells. The results indicate the importance of E7 in the immortalization of primary keratinocytes but suggest that the binding of pRB is not essential.

MeSH Terms
Amino Acid Sequence Animals Cell Transformation, Viral DNA Mutational Analysis Humans Keratinocytes/microbiology Molecular Sequence Data Oncogene Proteins, Viral/chemistry,metabolism Papillomaviridae/pathogenicity Papillomavirus E7 Proteins Protein Binding Retinoblastoma Protein/metabolism Structure-Activity Relationship Zinc Fingers
Chemicals
Oncogene Proteins, Viral Papillomavirus E7 Proteins Retinoblastoma Protein oncogene protein E7, Human papillomavirus type 16
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jewers R J
Richard Dimbleby Laboratory for Cancer Virology, UMDS, St. Thomas' Hospital, London, England.
Hildebrandt P
Ludlow J W
Kell B
McCance D J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.3.1329-1335.1992
Published
1992-03-00
Pages
1329-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240854
Subset
IM
Grants
NIAID NIH HHS · AI30798-02 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.3.1329-1335.1992 PMC Full Text View journal details
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