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PMID: 1309911 Published · ppublish English Journal Article

Influence of adeno-associated virus on adherence and growth properties of normal cells.

Journal of virology ·Vol. 66 ·No. 2 ·1992-02-00 ·Pages 773-9

Bantel-Schaal U, Stöhr M

Abstract

It has been shown previously that infection of newly established cell cultures from malignant human tumors with adeno-associated parvovirus type 2 or type 5 results in growth arrest and cell death. Here we report that the additionally observed antiproliferative effect on diploid human fibroblasts is transient and is connected to a reduced number of cells in S phase. Progression through the cell cycle is disturbed either in G0/G1 or at the G1/S boundary, but an additional arrest in G2 cannot be excluded. DNA synthesis and cell proliferation are resumed when cells are recultured after loosening of cell-matrix adhesions by trypsin treatment. In contrast, they are not resumed by solely providing growth factors via higher amounts of fetal calf serum. The results suggest that cell adherence is altered in adeno-associated parvovirus-infected human embryo fibroblasts.

MeSH Terms
Cell Adhesion Cell Cycle Cell Division/drug effects Cell Transformation, Viral Cells, Cultured DNA Replication Dependovirus/genetics Edetic Acid/pharmacology Embryo, Mammalian Fibroblasts/cytology,drug effects Flow Cytometry HeLa Cells Humans Trypsin/pharmacology
Chemicals
Edetic Acid Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bantel-Schaal U
Deutsches Krebsforschungszentrum Heidelberg, Institut für Virusforschung, Germany.
Stöhr M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.2.773-779.1992
Published
1992-02-00
Pages
773-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240777
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.2.773-779.1992 PMC Full Text View journal details
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