Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 6 ·1974-06-00 ·Pages 2432-4

Brown RL, Stubblefield E

Abstract

DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.

MeSH Terms

Animals Cell Line Cell-Free System Chymotrypsin/pharmacology Cricetinae DNA/biosynthesis Fibroblasts Kinetics Pronase/pharmacology Stimulation, Chemical Trypsin/pharmacology

Chemicals

DNA Chymotrypsin Trypsin Pronase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brown R L

Stubblefield E

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.6.2432

Published

1974-06-00

Pages

2432-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388471

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.6.2432 PMC Full Text View journal details

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Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.

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