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PMID: 12949117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Disparate oxygen responsiveness of two regulatory cascades that control expression of symbiotic genes in Bradyrhizobium japonicum.

Journal of bacteriology ·Vol. 185 ·No. 18 ·2003-09-00 ·Pages 5639-42

Sciotti MA, Chanfon A, Hennecke H, Fischer HM

Abstract

Two oxygen-responsive regulatory systems controlling numerous symbiotic genes in Bradyrhizobium japonicum were assayed in free-living cultures for their capacity to activate target genes under different oxygen conditions. NifA- and FixLJ-controlled target genes showed disparate relative expression patterns. Induction of NifA-dependent genes was observed only at oxygen concentrations below 2% in the gas phase, whereas that of FixLJ-controlled targets progressively increased when the oxygen concentration was lowered from 21 to 5, 2, or 0.5%. We propose that this reflects a response to a gradient of increasing oxygen deprivation as bacteria invade their host during root nodule development.

MeSH Terms
Bacterial Proteins/genetics,metabolism Bradyrhizobium/genetics,metabolism Gene Expression Regulation, Bacterial Hemeproteins/genetics,metabolism Histidine Kinase Kinetics Oxygen/metabolism Plant Roots/microbiology Recombinant Fusion Proteins/genetics,metabolism Symbiosis/genetics Transcription Factors/genetics,metabolism
Chemicals
Bacterial Proteins Hemeproteins NifA protein, Bacteria Recombinant Fusion Proteins Transcription Factors FixJ protein, Bacteria FixL protein, Bacteria Histidine Kinase Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sciotti Michel-Angelo
Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
Chanfon Astrid
Hennecke Hauke
Fischer Hans-Martin
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.18.5639-5642.2003
Published
2003-09-00
Pages
5639-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC193759
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.18.5639-5642.2003 PMC Full Text View journal details
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