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PMID: 9748464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bradyrhizobium japonicum FixK2, a crucial distributor in the FixLJ-dependent regulatory cascade for control of genes inducible by low oxygen levels.

Journal of bacteriology ·Vol. 180 ·No. 19 ·1998-10-00 ·Pages 5251-5

Nellen-Anthamatten D, Rossi P, Preisig O, Kullik I, Babst M, Fischer HM, Hennecke H

Abstract

Bradyrhizobium japonicum possesses a second fixK-like gene, fixK2, in addition to the previously identified fixK1 gene. The expression of both genes depends in a hierarchical fashion on the low-oxygen-responsive two-component regulatory system FixLJ, whereby FixJ first activates fixK2, whose product then activates fixK1. While the target genes for control by FixK1 are unknown, there is evidence for activation of the fixNOQP, fixGHIS, and rpoN1 genes and some heme biosynthesis and nitrate respiration genes by FixK2. FixK2 also regulates its own structural gene, directly or indirectly, in a negative way.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,physiology Base Sequence Gene Expression Regulation, Bacterial/physiology Genes, Bacterial/genetics Hemeproteins/physiology Histidine Kinase Models, Genetic Molecular Sequence Data Mutation Operon/genetics Oxygen Recombinant Fusion Proteins Restriction Mapping Rhizobiaceae/genetics,metabolism Sequence Homology, Amino Acid Transcription, Genetic/genetics Transcriptional Activation/genetics
Chemicals
Bacterial Proteins Hemeproteins Recombinant Fusion Proteins FixK protein, Bacteria FixJ protein, Bacteria FixL protein, Bacteria Histidine Kinase Oxygen
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nellen-Anthamatten D
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
Rossi P
Preisig O
Kullik I
Babst M
Fischer H M
Hennecke H
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.19.5251-5255.1998
Published
1998-10-00
Pages
5251-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107567
Subset
IM
Databases
GENBANK
AJ005001
External Links
PubMed source DOI: 10.1128/JB.180.19.5251-5255.1998 PMC Full Text View journal details
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