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PMID: 12941892 Published · ppublish English Journal Article

Enhanced disease and pulmonary eosinophilia associated with formalin-inactivated respiratory syncytial virus vaccination are linked to G glycoprotein CX3C-CX3CR1 interaction and expression of substance P.

Journal of virology ·Vol. 77 ·No. 18 ·2003-09-00 ·Pages 9831-44

Haynes LM, Jones LP, Barskey A, Anderson LJ, Tripp RA

Abstract

Vaccination with formalin-inactivated respiratory syncytial virus (FI-RSV) vaccine or RSV G glycoprotein results in enhanced pulmonary disease after live RSV infection. Enhanced pulmonary disease is characterized by pulmonary eosinophilia and is associated with a substantial inflammatory response. We show that the absence of the G glycoprotein or G glycoprotein CX3C motif during FI-RSV vaccination or RSV challenge of FI-RSV-vaccinated mice, or treatment with anti-substance P or anti-CX3CR1 antibodies, reduces or eliminates enhanced pulmonary disease, modifies T-cell receptor Vbeta usage, and alters CC and CXC chemokine expression. These data suggest that the G glycoprotein, and in particular the G glycoprotein CX3C motif, is key in the enhanced inflammatory response to FI-RSV vaccination, possibly through the induction of substance P.

MeSH Terms
Amino Acid Motifs Animals CD4-Positive T-Lymphocytes/immunology CX3C Chemokine Receptor 1 Cell Movement Chemokines/genetics Chemokines, CX3C/metabolism Female Formaldehyde Membrane Proteins Mice Mice, Inbred BALB C Pulmonary Eosinophilia/etiology RNA, Messenger/analysis Receptors, Antigen, T-Cell, alpha-beta/physiology Receptors, Chemokine/metabolism Respiratory Syncytial Viruses/immunology Substance P/biosynthesis Vaccination/adverse effects Vaccines, Inactivated/toxicity Viral Proteins/chemistry,physiology Viral Vaccines/toxicity
Chemicals
CX3C Chemokine Receptor 1 CX3CR1 protein, human Chemokines Chemokines, CX3C Membrane Proteins RNA, Messenger Receptors, Antigen, T-Cell, alpha-beta Receptors, Chemokine Vaccines, Inactivated Viral Proteins Viral Vaccines Formaldehyde Substance P
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haynes Lia M
Division of Viral and Rickettsial Diseases, National Center of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Jones Les P
Barskey Albert
Anderson Larry J
Tripp Ralph A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.18.9831-9844.2003
Published
2003-09-00
Pages
9831-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC224581
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.18.9831-9844.2003 PMC Full Text View journal details
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