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PMID: 12861016 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos.

Molecular and cellular biology ·Vol. 23 ·No. 15 ·2003-08-00 ·Pages 5301-7

Cao S, Bendall H, Hicks GG, Nashabi A, Sakano H, Shinkai Y, Gariglio M, Oltz EM, Ruley HE

Abstract

The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.

MeSH Terms
Alleles Animals Apoptosis Blotting, Southern Blotting, Western Cell Division Cell Survival DNA-Binding Proteins/chemistry,physiology Genes, p53 Genetic Vectors Genotype High Mobility Group Proteins/chemistry,physiology Homozygote In Situ Nick-End Labeling Mice Models, Genetic Mutation Recombination, Genetic Stem Cells/metabolism Time Factors
Chemicals
DNA-Binding Proteins High Mobility Group Proteins Ssrp1 protein, mouse
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Cao Shang
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, 1161 21st Avenue South, AA 4210 MCN, Nashville, TN 37232-2363, USA.
Bendall Heather
Hicks Geoffrey G
Nashabi Abudi
Sakano Hitoshi
Shinkai Yoichi
Gariglio Marisa
Oltz Eugene M
Ruley H Earl
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.15.5301-5307.2003
Published
2003-08-00
Pages
5301-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC165710
Subset
IM
Grants
NHLBI NIH HHS · P01 HL 68744 · United States
NIAID NIH HHS · R37 AI 18790 · United States
NHLBI NIH HHS · P01 HL068744 · United States
NCI NIH HHS · P30 CA 68485 · United States
NCI NIH HHS · P30 CA068485 · United States
External Links
PubMed source DOI: 10.1128/MCB.23.15.5301-5307.2003 PMC Full Text View journal details
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