A direct role for GRASP65 as a mitotically regulated Golgi stacking factor.
Cell-free assays that mimic the disassembly and reassembly cycle of the Golgi apparatus during mitosis implicated GRASP65 as a mitotically regulated stacking factor. We now present evidence that GRASP65 is directly involved in stacking Golgi cisternae. GRASP65 is the major phosphorylation target in rat liver Golgi membranes of two mitotic kinases, cdc2-cyclin B and polo-like kinases, which alone will unstack Golgi membranes, generating single cisternae. Mitotic cells microinjected with antibodies to GRASP65 fail to form proper Golgi stacks after cell division. Beads coated with GRASP65 homodimers form extensive aggregates consistent with the formation of trans oligomers. These can be disaggregated using purified cdc2-cyclin B1 and polo-like kinases, and re-aggregated after dephosphorylation of GRASP65. Together, these data demonstrate that GRASP65 has the properties required to bind surfaces together in a mitotically regulated manner.
26 references, click to expand
-
A role for the vesicle tethering protein, p115, in the post-mitotic stacking of reassembling Golgi cisternae in a cell-free system.J Cell Biol. 1999 Jul 12;146(1):57-70 PMID: 10402460
-
Genetic selection of peptide inhibitors of biological pathways.Science. 1999 Jul 23;285(5427):591-5 PMID: 10417390
-
GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system.EMBO J. 1999 Sep 15;18(18):4949-60 PMID: 10487747
-
The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo.Mol Biol Cell. 2000 Feb;11(2):635-45 PMID: 10679020
-
Phosphorylation of the vesicle-tethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments.J Cell Biol. 2000 Aug 7;150(3):475-88 PMID: 10931861
-
Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase (Plk) and Cdc2.Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12589-94 PMID: 11050165
-
Transmembrane transforming growth factor-alpha tethers to the PDZ domain-containing, Golgi membrane-associated protein p59/GRASP55.EMBO J. 2000 Dec 1;19(23):6427-39 PMID: 11101516
-
The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus.J Cell Biol. 2001 Mar 5;152(5):945-58 PMID: 11238451
-
Mitotic phosphorylation of Golgi reassembly stacking protein 55 by mitogen-activated protein kinase ERK2.Mol Biol Cell. 2001 Jun;12(6):1811-7 PMID: 11408587
-
Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis.Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9128-32 PMID: 11447294
-
Constructing a Golgi complex.J Cell Biol. 2001 Dec 10;155(6):873-5 PMID: 11739400
-
A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic.J Cell Biol. 2001 Dec 10;155(6):877-83 PMID: 11739401
-
Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus.J Cell Biol. 2001 Dec 10;155(6):885-91 PMID: 11739402
-
Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis.J Cell Biol. 2002 Feb 4;156(3):495-509 PMID: 11815631
-
Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.Cell. 2002 May 3;109(3):359-69 PMID: 12015985
-
The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage.J Cell Biol. 1981 Dec;91(3 Pt 2):77s-103s PMID: 7033246
-
Assembly of asparagine-linked oligosaccharides.Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
-
The Golgi stack reassembles during telophase before arrival of proteins transported from the endoplasmic reticulum.J Cell Biol. 1993 Aug;122(3):533-40 PMID: 8335684
-
Reassembly of Golgi stacks from mitotic Golgi fragments in a cell-free system.J Cell Biol. 1995 May;129(3):605-18 PMID: 7730399
-
A role for tubular networks and a COP I-independent pathway in the mitotic fragmentation of Golgi stacks in a cell-free system.J Cell Biol. 1995 Sep;130(5):1027-39 PMID: 7657690
-
An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments.Cell. 1995 Sep 22;82(6):905-14 PMID: 7553851
-
Cyclin/Cdk-dependent initiation of DNA replication in a human cell-free system.Cell. 1997 Jan 10;88(1):109-19 PMID: 9019396
-
GRASP65, a protein involved in the stacking of Golgi cisternae.Cell. 1997 Oct 17;91(2):253-62 PMID: 9346242
-
A role for giantin in docking COPI vesicles to Golgi membranes.J Cell Biol. 1998 Mar 9;140(5):1013-21 PMID: 9490716
-
Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae.EMBO J. 1998 Jun 15;17(12):3258-68 PMID: 9628863
-
Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis.Cell. 1998 Sep 18;94(6):783-93 PMID: 9753325
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