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PMID: 11739401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic.

The Journal of cell biology ·Vol. 155 ·No. 6 ·2001-12-10 ·Pages 877-83

Short B, Preisinger C, Körner R, Kopajtich R, Byron O, Barr FA

Abstract

Membrane traffic between the endoplasmic reticulum (ER) and Golgi apparatus and through the Golgi apparatus is a highly regulated process controlled by members of the rab GTPase family. The GTP form of rab1 regulates ER to Golgi transport by interaction with the vesicle tethering factor p115 and the cis-Golgi matrix protein GM130, also part of a complex with GRASP65 important for the organization of cis-Golgi cisternae. Here, we find that a novel coiled-coil protein golgin-45 interacts with the medial-Golgi matrix protein GRASP55 and the GTP form of rab2 but not other Golgi rab proteins. Depletion of golgin-45 disrupts the Golgi apparatus and causes a block in secretory protein transport. These results demonstrate that GRASP55 and golgin-45 form a rab2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure.

MeSH Terms
Animals Autoantigens Golgi Apparatus/chemistry,enzymology,ultrastructure Golgi Matrix Proteins HeLa Cells Humans Kidney/cytology Membrane Proteins/analysis,genetics,metabolism Mice Molecular Sequence Data Protein Transport/physiology Rats Two-Hybrid System Techniques Yeasts rab2 GTP-Binding Protein/analysis,metabolism
Chemicals
Autoantigens GORASP1 protein, human GORASP2 protein, human Golgi Matrix Proteins Golgin subfamily A member 2 Gorasp1 protein, rat Membrane Proteins rab2 GTP-Binding Protein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Short B
Department of Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried D-82152, Germany.
Preisinger C
Körner R
Kopajtich R
Byron O
Barr F A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200108079
Published
2001-12-10
Epub
2001-00-10
Pages
877-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2150909
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
AK006544
Corrections
CommentIn
PMID: 11739400
External Links
PubMed source DOI: 10.1083/jcb.200108079 PMC Full Text View journal details
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