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PMID: 12829857 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The zipper region of Epstein-Barr virus bZIP transcription factor Zta is necessary but not sufficient to direct DNA binding.

Journal of virology ·Vol. 77 ·No. 14 ·2003-07-00 ·Pages 8173-7

Hicks MR, Al-Mehairi SS, Sinclair AJ

Abstract

The viral bZIP transcription factor Zta (BZLF1, EB1, ZEBRA) mediates the switch between the latent and lytic cycles of Epstein-Barr virus (EBV). In part, its activity requires the formation of homodimers and interaction with specific DNA sequence elements (ZREs). Zta has an atypical zipper motif that has a lower stability than do typical bZIP proteins. Here we show that a synthetic peptide directed against the zipper can disrupt the DNA-binding function of Zta. This highlights the relevance of this region for the function of Zta and demonstrates that the zipper region is a potential target for therapeutic agents. We also unmask the relevance of a region adjacent to the zipper (CT region), which is required to direct the interaction of Zta with DNA and to transactivate ZRE-dependent promoters in vivo.

MeSH Terms
Amino Acid Sequence DNA/metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Dimerization Gene Expression Regulation, Viral HeLa Cells Herpesvirus 4, Human/metabolism Humans Leucine Zippers Molecular Sequence Data Trans-Activators/chemistry,genetics,metabolism Transfection Viral Proteins
Chemicals
BZLF1 protein, Herpesvirus 4, Human DNA-Binding Proteins Trans-Activators Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hicks Matthew R
School of Biological Sciences, University of Sussex, Brighton, East Sussex BN1 9QG, United Kingdom.
Al-Mehairi Salama S
Sinclair Alison J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.14.8173-8177.2003
Published
2003-07-00
Pages
8173-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC161931
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.14.8173-8177.2003 PMC Full Text View journal details
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