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PMID: 12824425 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

SIFT: Predicting amino acid changes that affect protein function.

Nucleic acids research ·Vol. 31 ·No. 13 ·2003-07-01 ·Pages 3812-4

Ng PC, Henikoff S

Abstract

Single nucleotide polymorphism (SNP) studies and random mutagenesis projects identify amino acid substitutions in protein-coding regions. Each substitution has the potential to affect protein function. SIFT (Sorting Intolerant From Tolerant) is a program that predicts whether an amino acid substitution affects protein function so that users can prioritize substitutions for further study. We have shown that SIFT can distinguish between functionally neutral and deleterious amino acid changes in mutagenesis studies and on human polymorphisms. SIFT is available at http://blocks.fhcrc.org/sift/SIFT.html.

MeSH Terms

Amino Acid Substitution Humans Internet Polymorphism, Genetic Proteins/chemistry,physiology Sequence Alignment Sequence Analysis, Protein/methods Software User-Computer Interface

Chemicals

Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ng Pauline C

Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N A1-162, Seattle, WA 98109, USA.

Henikoff Steven

References (13)

13 references, click to expand

The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000.
Nucleic Acids Res. 2000 Jan 1;28(1):45-8 PMID: 10592178
Human gene mutation database-a biomedical information and research resource.
Hum Mutat. 2000;15(1):45-51 PMID: 10612821
dbSNP: the NCBI database of genetic variation.
Nucleic Acids Res. 2001 Jan 1;29(1):308-11 PMID: 11125122
Prediction of deleterious human alleles.
Hum Mol Genet. 2001 Mar 15;10(6):591-7 PMID: 11230178
Predicting the functional consequences of non-synonymous single nucleotide polymorphisms: structure-based assessment of amino acid variation.
J Mol Biol. 2001 Mar 23;307(2):683-706 PMID: 11254390
Predicting deleterious amino acid substitutions.
Genome Res. 2001 May;11(5):863-74 PMID: 11337480
Lac repressor genetic map in real space.
Trends Biochem Sci. 1997 Sep;22(9):334-9 PMID: 9301333
Accounting for human polymorphisms predicted to affect protein function.
Genome Res. 2002 Mar;12(3):436-46 PMID: 11875032
Human non-synonymous SNPs: server and survey.
Nucleic Acids Res. 2002 Sep 1;30(17):3894-900 PMID: 12202775
Evaluation of structural and evolutionary contributions to deleterious mutation prediction.
J Mol Biol. 2002 Sep 27;322(4):891-901 PMID: 12270722
Natural variation in human membrane transporter genes reveals evolutionary and functional constraints.
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5896-901 PMID: 12719533
Information content of binding sites on nucleotide sequences.
J Mol Biol. 1986 Apr 5;188(3):415-31 PMID: 3525846
Database resources of the National Center for Biotechnology Information: 2002 update.
Nucleic Acids Res. 2002 Jan 1;30(1):13-6 PMID: 11752242

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

1362-4962

DOI

10.1093/nar/gkg509

Published

2003-07-01

Pages

3812-4

Language

English

Region

England

NLM ID

0411011

PMCID

PMC168916

Subset

Grants

NIGMS NIH HHS · GM29009 · United States

External Links

PubMed source DOI: 10.1093/nar/gkg509 PMC Full Text View journal details

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