Home LiteratureArticle Details

PMID: 12820726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tumor cell MUC1 and CD43 are glycosylated differently with sialyl-Lewis a and x epitopes and show variable interactions with E-selectin under physiological flow conditions.

Glycoconjugate journal ·Vol. 18 ·No. 11-12 ·2001-00-00 ·Pages 925-30

Fernandez-Rodriguez J, Dwir O, Alon R, Hansson GC

Abstract

The mucins secreted from the colon carcinoma cell line COLO 205 have the MUC1 and CD43 (leukosialin) as core proteins, where both carry sialyl-Lewis a and MUC1 sialyl-Lewis x epitopes. The adhesion of E-selectin expressing CHO cells to the coated mucins was analyzed in a flow system revealing that the MUC1 mucin adhered better than the CD43 mucin. One reason could be their different glycosylation, a difference that was explored by analyzing the biosynthesis of MUC1 and CD43 in COLO 205 cells. Both the MUC1 and CD43 mucins became sialyl-Lewis a reactive, but after different times as revealed by pulse-chase studies. However, only MUC1 became sialyl-Lewis x reactive. These differences suggest that MUC1 and CD43 are synthesized in different compartments of the cell. It was also observed that the mucins from colon carcinoma patients had MUC1-type mucins that carried both sialyl-Lewis a and x epitopes and CD43-type sialyl-Lewis a mucins with only low levels of sialyl-Lewis x epitopes. One could hypothesize that colon carcinoma derived MUC1 is decorated with potent E-selectin epitopes, and that this could be one of several reasons for the involvement of MUC1 in cancer development.

MeSH Terms

Adenocarcinoma/immunology,metabolism Animals Antigens, CD CA-19-9 Antigen CHO Cells/metabolism Carbohydrate Sequence Cell Adhesion/physiology Colonic Neoplasms/immunology,metabolism Cricetinae E-Selectin/genetics,metabolism Epitopes/metabolism Gangliosides/immunology Glycosylation HL-60 Cells/pathology Humans Leukosialin Molecular Sequence Data Mucin-1/immunology,metabolism Oligosaccharides/immunology Rabbits Sialoglycoproteins/immunology,metabolism Sialyl Lewis X Antigen Stress, Mechanical Tumor Cells, Cultured

Chemicals

Antigens, CD CA-19-9 Antigen E-Selectin Epitopes Gangliosides Leukosialin Mucin-1 Oligosaccharides SPN protein, human Sialoglycoproteins Sialyl Lewis X Antigen sialyl Le(a) ganglioside

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fernandez-Rodriguez J

Department of Medical Biochemistry, Göteborg University, Box 440, 405 30 Gothenburg, Sweden.

Dwir O

Alon R

Hansson G C

References (27)

27 references, click to expand

Increased expression of sialyl Lewisx antigen correlates with poor survival in patients with colorectal carcinoma: clinicopathological and immunohistochemical study.
Cancer Res. 1993 Aug 1;53(15):3632-7 PMID: 8101764
Serum CA 19-9 concentrations and computed tomography findings in patients with pancreatic carcinoma.
Cancer. 1986 Apr 1;57(7):1324-6 PMID: 3456252
Expression of the leukocyte-associated sialoglycoprotein CD43 by a colon carcinoma cell line.
J Biol Chem. 1995 Jun 9;270(23):13688-92 PMID: 7775421
MUC1, the renaissance molecule.
J Mammary Gland Biol Neoplasia. 2001 Jul;6(3):339-53 PMID: 11547902
Secreted MUC1 mucins lacking their cytoplasmic part and carrying sialyl-Lewis a and x epitopes from a tumor cell line and sera of colon carcinoma patients can inhibit HL-60 leukocyte adhesion to E-selectin-expressing endothelial cells.
J Cell Biochem. 1996 Mar 15;60(4):538-49 PMID: 8707893
Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.
Cell. 1991 May 31;65(5):859-73 PMID: 1710173
A secreted mucin carrying sialyl-Lewis a from colon carcinoma cells binds to E-selectin and inhibits HL-60 cell adhesion.
Int J Cancer. 1994 Dec 15;59(6):823-9 PMID: 7527376
Rolling of lymphocytes and neutrophils on peripheral node addressin and subsequent arrest on ICAM-1 in shear flow.
Eur J Immunol. 1995 Apr;25(4):1025-31 PMID: 7537667
A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.
J Biol Chem. 1982 Dec 10;257(23):14365-9 PMID: 7142214
Difference in prognostic value between sialyl Lewis(a) and sialyl lewis(x) antigens in blood samples obtained from the drainage veins of the colorectal tumors.
Cancer Lett. 2000 Oct 31;159(2):159-68 PMID: 10996727
Expression and functional characterization of a soluble form of vascular cell adhesion molecule 1.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):1498-504 PMID: 1714725
A carbohydrate domain common to both sialyl Le(a) and sialyl Le(X) is recognized by the endothelial cell leukocyte adhesion molecule ELAM-1.
J Biol Chem. 1991 Aug 15;266(23):14869-72 PMID: 1714447
Colon adenoma and cancer cells aberrantly express the leukocyte-associated sialoglycoprotein CD43.
Biochem Biophys Res Commun. 1997 Sep 18;238(2):612-6 PMID: 9299561
Quantitative and qualitative characterization of human cancer-associated serum glycoprotein antigens expressing fucosyl or sialyl-fucosyl type 2 chain polylactosamine.
Cancer Res. 1986 May;46(5):2619-26 PMID: 3008996
Purification and characterization of a membrane-bound and a secreted mucin-type glycoprotein carrying the carcinoma-associated sialyl-Lea epitope on distinct core proteins.
J Biol Chem. 1991 Nov 15;266(32):21537-47 PMID: 1718981
Comparison of serological expression of different epitopes on the CA50-carrying antigen CanAg.
Int J Cancer. 1991 Jul 9;48(5):757-63 PMID: 1712758
MUC1 and cancer.
Biochim Biophys Acta. 1999 Oct 8;1455(2-3):301-13 PMID: 10571020
Circulating sialyl Lewis(x), sialyl Lewis(a), and sialyl Tn antigens in colorectal cancer patients: multivariate analysis of predictive factors for serum antigen levels.
J Gastroenterol. 2001 Mar;36(3):166-72 PMID: 11291879
Mouse monoclonal antibodies for experimental immunotherapy promotes killing of tumor cells.
Int J Cancer. 1988 Dec 15;42(6):877-82 PMID: 3192334
C-type lectins and sialyl Lewis X oligosaccharides. Versatile roles in cell-cell interaction.
J Cell Biol. 1999 Nov 1;147(3):467-70 PMID: 10545492
Biosynthesis of the cancer-associated sialyl-Lea antigen.
J Biol Chem. 1985 Aug 5;260(16):9388-92 PMID: 4019478
Post-translational fate of a mucin-like leukocyte sialoglycoprotein (CD43) aberrantly expressed in a colon carcinoma cell line.
J Biol Chem. 1997 Apr 25;272(17):11503-9 PMID: 9111064
Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin.
Cancer Res. 1983 Nov;43(11):5489-92 PMID: 6193872
Comparison of sialyl-Lewis a-carrying CD43 and MUC1 mucins secreted from a colon carcinoma cell line for E-selectin binding and inhibition of leukocyte adhesion.
Tumour Biol. 1997;18(3):175-87 PMID: 9143414
Dual roles of sialyl Lewis X oligosaccharides in tumor metastasis and rejection by natural killer cells.
EMBO J. 1999 Mar 15;18(6):1516-25 PMID: 10075923
An endothelial ligand for L-selectin is a novel mucin-like molecule.
Cell. 1992 Jun 12;69(6):927-38 PMID: 1376638
GlyCAM-1 supports leukocyte rolling in flow: evidence for a greater dynamic stability of L-selectin rolling of lymphocytes than of neutrophils.
Cell Adhes Commun. 1998;6(4):349-70 PMID: 9865468

Article Info

Journal

Glycoconjugate journal

Abbr.

Glycoconj J

ISSN

0282-0080

DOI

10.1023/a:1022208727512

Published

2001-00-00

Pages

925-30

Language

English

Region

United States

NLM ID

8603310

Subset

External Links

PubMed source DOI: 10.1023/a:1022208727512 View journal details

Analysis Services

Tumor cell MUC1 and CD43 are glycosylated differently with sialyl-Lewis a and x epitopes and show variable interactions with E-selectin under physiological flow conditions.

Contact

WeChat Official Account