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PMID: 12805221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Schizosaccharomyces pombe AGC family kinase Gad8p forms a conserved signaling module with TOR and PDK1-like kinases.

The EMBO journal ·Vol. 22 ·No. 12 ·2003-06-16 ·Pages 3073-83

Matsuo T, Kubo Y, Watanabe Y, Yamamoto M

Abstract

The TOR protein is a phosphoinositide kinase-related kinase widely conserved among eukaryotes. Fission yeast tor1 encodes an ortholog of TOR, which is required for sexual development and growth under stressed conditions. We isolated gad8, which encodes a Ser/Thr kinase of the AGC family, as a high-copy suppressor of the sterility of a tor1 mutant. Disruption of gad8 caused phenotypes similar to those of tor1 disruption. Gad8p was less phosphorylated and its kinase activity was undetectable in tor1Delta cells. Three amino acid residues corresponding to conserved phosphorylation sites in the AGC family kinases, namely Thr387 in the activation loop, Ser527 in the turn motif and Ser546 in the hydrophobic motif, were important for the kinase activity of Gad8p. Tor1p was responsible for the phosphorylation of Ser527 and Ser546, whereas Ksg1p, a PDK1-like kinase, appeared to phosphorylate Thr387 directly. Altogether, Tor1p, Ksg1p and Gad8p appear to constitute a signaling module for sexual development and growth under stressed conditions in fission yeast, which resembles the mTOR-PDK1-S6K1 system in mammals and may represent a basic signaling module ubiquitous in eukaryotes.

MeSH Terms
3-Phosphoinositide-Dependent Protein Kinases Animals Humans Mutation Phosphatidylinositol 3-Kinases/genetics Phosphorylation Phosphotransferases (Alcohol Group Acceptor)/genetics Protein Kinases/metabolism Protein Serine-Threonine Kinases/chemistry,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics Schizosaccharomyces/enzymology,physiology Schizosaccharomyces pombe Proteins/chemistry,genetics,metabolism Serine/metabolism Signal Transduction/physiology
Chemicals
Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Serine Protein Kinases Phosphotransferases (Alcohol Group Acceptor) TOR1 protein, S cerevisiae 3-Phosphoinositide-Dependent Protein Kinases Gad8 protein, S pombe KSG1 protein, S pombe PDPK1 protein, human Protein Serine-Threonine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matsuo Tomohiko
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
Kubo Yoshiya
Watanabe Yoshinori
Yamamoto Masayuki
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg298
Published
2003-06-16
Pages
3073-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC162150
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg298 PMC Full Text View journal details
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