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PMID: 1280322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene.

Molecular and cellular biology ·Vol. 12 ·No. 12 ·1992-12-00 ·Pages 5415-25

Gallego ME, Balvay L, Brody E

Abstract

The chicken beta-tropomyosin gene contains an internal pair of mutually exclusive exons (6A and 6B) that are selected in a tissue-specific manner. Exon 6A is incorporated in fibroblasts and smooth muscle cells, whereas exon 6B is skeletal muscle specific. In this study we show that two different regions in the intron between the two mutually exclusive exons are important for this specific selection in nonmuscle cells. Sequences in the 3' end of the intron have a negative effect in the recognition of the 3' splice site, while sequences in the 5' end of the intron have a positive effect in the recognition of the 5' splice site. First, sequences in exon 6B as well as in the intron upstream of exon 6B are both able to inhibit splicing when placed in a heterologous gene. The sequences in the polypyrimidine stretch region contribute to splicing inhibition of exons 5 or 6A to 6B through a mechanism independent of their implication in the previously described secondary structure around exon 6B. Second, we have identified a sequence of 30 nucleotides in the intron just downstream of exon 6A that is essential for the recognition of the 5' splice site of exon 6A. This is so even after introduction of a consensus sequence into the 5' splice site of this exon. Deletion of this sequence blocks splicing of exon 6A to 6B after formation of the presplicing complex. Taken together, these results suggest that both the mutually exclusive behavior and the choice between exons 6A and 6B of the chicken beta-tropomyosin gene are trans regulated.

MeSH Terms
Animals Base Sequence Chickens Cloning, Molecular DNA Exons HeLa Cells Humans Introns Molecular Sequence Data Nucleic Acid Conformation RNA RNA Splicing Regulatory Sequences, Nucleic Acid Tropomyosin/genetics
Chemicals
Tropomyosin RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gallego M E
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Balvay L
Brody E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.12.5415-5425.1992
Published
1992-12-00
Pages
5415-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360479
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.12.12.5415-5425.1992 PMC Full Text View journal details
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