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PMID: 12796297 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Novel Rho GTPase involved in cytokinesis and cell wall integrity in the fission yeast Schizosaccharomyces pombe.

Eukaryotic cell ·Vol. 2 ·No. 3 ·2003-06-00 ·Pages 521-33

Santos B, Gutiérrez J, Calonge TM, Pérez P

Abstract

The Rho family of GTPases is present in all eukaryotic cells from yeast to mammals; they are regulators in signaling pathways that control actin organization and morphogenetic processes. In yeast, Rho GTPases are implicated in cell polarity processes and cell wall biosynthesis. It is known that Rho1 and Rho2 are key proteins in the construction of the cell wall, an essential structure that in Schizosaccharomyces pombe is composed of beta-glucan, alpha-glucan, and mannoproteins. Rho1 regulates the synthesis of 1,3-beta-D-glucan by activation of the 1,3-beta-D-glucan synthase, and Rho2 regulates the synthesis of alpha-glucan by the 1,3-alpha-D-glucan synthase Mok1. Here we describe the characterization of another Rho GTPase in fission yeast, Rho4. rho4Delta cells are viable but display cell separation defects at high temperature. In agreement with this observation, Rho4 localizes to the septum. Overexpression of rho4(+) causes lysis and morphological defects. Several lines of evidence indicate that both rho4(+) deletion or rho4(+) overexpression result in a defective cell wall, suggesting an additional role for Rho4 in cell wall integrity. Rho4Delta cells also accumulate secretory vesicles around the septum and are defective in actin polarization. We propose that Rho4 could be involved in the regulation of the septum degradation during cytokinesis.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence Cell Division/physiology Cell Wall/metabolism Gene Deletion Genes, Fungal Green Fluorescent Proteins Luminescent Proteins/metabolism Molecular Sequence Data Mutation Schizosaccharomyces Schizosaccharomyces pombe Proteins/chemistry,genetics,physiology,ultrastructure Sequence Homology, Amino Acid Temperature rho GTP-Binding Proteins/chemistry,genetics,physiology,ultrastructure
Chemicals
Luminescent Proteins Schizosaccharomyces pombe Proteins Green Fluorescent Proteins rho GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Santos Beatriz
Instituto Microbiología-Bioquímica, Departamento de Microbiología-Genética, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007 Salamanca, Spain. bsr@usal.es
Gutiérrez Javier
Calonge Teresa M
Pérez Pilar
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.2.3.521-533.2003
Email
bsr@usal.es
Published
2003-06-00
Pages
521-33
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC161461
Subset
IM
External Links
PubMed source DOI: 10.1128/EC.2.3.521-533.2003 PMC Full Text View journal details
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