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PMID: 12775413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of CCR2, CCR5, and CXCR3 by CD4+ T cells is stable during a 2-year longitudinal study but varies widely between individuals.

Journal of neurovirology ·Vol. 9 ·No. 3 ·2003-06-00 ·Pages 291-9

Kivisäkk P, Trebst C, Lee JC, Tucky BH, Rudick RA, Campbell JJ, Ransohoff RM

Abstract

Blockade of chemokine receptors (CKRs) has recently emerged as a possible pathway for therapeutic intervention in disease. In the present report, the expression of CCR2, CCR5, and CXCR3, associated with migration of mononuclear cells to inflamed tissue, was determined on CD4+ T cells in a 2-year longitudinal study of healthy volunteers using flow cytometry. Large interindividual variations in the expression of these receptors on CD4+ T cells were observed, whereas levels remained remarkably stable over time within subjects. The expression of CCR2, CCR5, and CXCR3 on CD4+ T cells was directly proportional to percentages of CD45RO(hi)/CD4+ T cells. In addition, highly significant associations between levels of CCR2, CCR5, and CXCR3 on CD4+ T cells were demonstrated in individual subjects, implying a common mechanism for regulating the expression of these CKRs on circulating T cells. These associations were not due to coexpression of CKRs on individual CD45RA-/CD4+ T cells. The results provide insight into the regulation of CKR expression on CD4+ T cells in vivo, and suggest that major fluctuations of CKR expression in individuals are uncommon.

MeSH Terms
Adult CD4 Antigens/analysis CD4-Positive T-Lymphocytes/metabolism Female Flow Cytometry Genetic Variation Humans Leukocyte Common Antigens/analysis Longitudinal Studies Male Middle Aged Receptors, CCR2 Receptors, CCR5/biosynthesis,genetics Receptors, CXCR3 Receptors, Chemokine/biosynthesis,genetics
Chemicals
CCR2 protein, human CD4 Antigens CXCR3 protein, human Receptors, CCR2 Receptors, CCR5 Receptors, CXCR3 Receptors, Chemokine Leukocyte Common Antigens
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kivisäkk Pia
Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Trebst Corinna
Lee Jar-Chi
Tucky Barbara H
Rudick Richard A
Campbell James J
Ransohoff Richard M
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Article Info
Journal
Journal of neurovirology
Abbr.
J Neurovirol
ISSN
1355-0284
DOI
10.1080/13550280390201001
Published
2003-06-00
Pages
291-9
Language
English
Region
United States
NLM ID
9508123
Subset
IM
Grants
NINDS NIH HHS · P01NS38667 · United States
External Links
PubMed source DOI: 10.1080/13550280390201001 View journal details
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