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PMID: 12771217 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.

Nucleic acids research ·Vol. 31 ·No. 11 ·2003-06-01 ·Pages 2900-14

Zhang X, Diab IH, Zehner ZE

Abstract

Vimentin, a member of the intermediate filament protein family, is regulated both developmentally and tissue specifically. It is also a marker of the metastatic potential of many tumor cells. Pre viously, the human vimentin promoter has been shown to contain multiple elements for the binding of both positive- and negative-acting regulatory factors. Transient transfection analysis of various vimentin 5'-end promoter sequences and mutants thereof fused to a reporter gene further defined two regulatory elements, a positive element that binds Sp1 and a negative element that binds the protein ZBP-89. ZBP-89 has been shown to be either a repressor or an activator of gene expression, depending on the promoter. Here, we show that for vimentin, both ZBP-89 and ZBP-99 repress reporter gene expression in Schneider (S2) cells. Deletion constructs confirm that the glutamine-rich region of Sp1 is required to enhance vimentin transcription, whereas the N-terminus of ZBP-89 is required to interact with Sp1 and repress gene expression. The overexpression of hTAF(II)130 can alleviate ZBP-89 repression in S2 cells, suggesting how ZBP-89 might serve to block gene expression.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins/genetics,metabolism,physiology Gene Silencing Glutamine/analysis HeLa Cells Humans Mutation Promoter Regions, Genetic Repressor Proteins/genetics,metabolism,physiology Response Elements Sequence Deletion Sp1 Transcription Factor/chemistry,genetics,metabolism Sp3 Transcription Factor TATA-Binding Protein Associated Factors/metabolism Trans-Activators Transcription Factor TFIID/metabolism Transcription Factors/genetics,metabolism,physiology Transcriptional Activation Vimentin/genetics
Chemicals
DNA-Binding Proteins Repressor Proteins Sp1 Transcription Factor TAF4 protein, human TATA-Binding Protein Associated Factors Trans-Activators Transcription Factor TFIID Transcription Factors Vimentin ZNF148 protein, human ZNF281 protein, human Glutamine Sp3 Transcription Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhang Xueping
Department of Biochemistry and the Massey Cancer Center, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, VA 23298-0614, USA.
Diab Iman H
Zehner Zendra E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkg380
Published
2003-06-01
Pages
2900-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC156715
Subset
IM
Grants
NHLBI NIH HHS · R01 HL045422 · United States
NHLBI NIH HHS · HL-45422 · United States
External Links
PubMed source DOI: 10.1093/nar/gkg380 PMC Full Text View journal details
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