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PMID: 12769287 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recent progress in chromosome painting of Arabidopsis and related species.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 11 ·No. 3 ·2003-00-00 ·Pages 195-204

Lysak MA, Pecinka A, Schubert I

Abstract

This paper reports on state-of-the-art achievements of chromosome painting in Arabidopsis thaliana (2n = 10). Arabidopsis chromosomes 1, 2 and 4 were painted using chromosome-specific BAC contigs. We consider technical aspects of the painting approach and document major applications, such as the tracing of Arabidopsis chromosomes as interphase chromosome territories and during mitotic and meiotic cell cycles as well as comparative chromosome painting in related species. This is the first report of successful interspecific chromosome painting in plants. The evolutionary history of chromosomes homeologous to Arabidopsis chromosome 4 was reconstructed by hybridization of chromosome-4-specific painting probes to karyotypes of Brassicaceae species with x = 8 chromosomes. Future perspectives of chromosome painting in A. thaliana and its wild relatives are outlined.

MeSH Terms
Arabidopsis/genetics Brassicaceae/genetics Cell Cycle Chromosome Painting/methods Chromosomes, Artificial, Bacterial/genetics Chromosomes, Plant/genetics Meiosis Mitosis Physical Chromosome Mapping
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lysak Martin A
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
Pecinka Ales
Schubert Ingo
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Article Info
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Abbr.
Chromosome Res
ISSN
0967-3849
DOI
10.1023/a:1022879608152
Published
2003-00-00
Pages
195-204
Language
English
Region
Netherlands
NLM ID
9313452
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1022879608152 View journal details
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