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PMID: 12734753 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Allelic imbalance regions on chromosomes 8p, 17p and 19p related to metastasis of hepatocellular carcinoma: comparison between matched primary and metastatic lesions in 22 patients by genome-wide microsatellite analysis.

Journal of cancer research and clinical oncology ·Vol. 129 ·No. 5 ·2003-05-00 ·Pages 279-86

Zhang LH, Qin LX, Ma ZC, Ye SL, Liu YK, Ye QH, Wu X, Huang W, Tang ZY

Abstract

To understand the molecular mechanisms of metastasis in hepatocellular carcinoma (HCC), it is necessary to identify the accumulating genetic alterations during its progression as well as those responsible for the acquisition of metastatic potential in cancer cells. In our previous study, using comparative genomic hybridization (CGH), we found that loss on chromosome 8p is more frequent in metastatic lesions than in matched primary tumors of HCC. Thus, 8p deletion might contribute to HCC metastasis. To narrow the location of metastasis-related alteration regions, we analyzed 22 primary and matched metastatic lesions of HCC by genome-wide microsatellite analysis. Common regions with high levels of allelic imbalance (AI) were identified on 17p, 8p11-cen, 8p21-23, 4q32-qter, 4q13-23, 16q, and 1p33. Regions with increased AI in metastatic lesions were 8p23.3, 8p11.2, 17p11.2-13.3, 4q21-22, 4q32-qter, 8q24.1, 9p11, 9q31, 11q23.1, 13q14.1-31, 13q32-qter, 16p13.3, 16q13, 16q22, and 19p13.1, and these were considered to be related to the metastasis phenotype. Among them, loss on 8p was again proved to be related to progression and metastasis of HCC, and 8p23.3 and 8p11.2 were two likely regions harboring metastasis-related genes. It was also shown for the first time in HCC that AI of 19p13.1 might also be related to metastatic potential. These results provide some candidate regions for further study to identify putative genes suppressing metastasis of HCC.

MeSH Terms
Adult Aged Allelic Imbalance Carcinoma, Hepatocellular/genetics,secondary Chromosomes, Human, Pair 17/genetics Chromosomes, Human, Pair 19/genetics Chromosomes, Human, Pair 8/genetics Disease Progression Female Humans Liver Neoplasms/genetics,secondary Male Microsatellite Repeats/genetics Middle Aged Polymerase Chain Reaction
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Zhang Lian-Hai
Liver Cancer Institute, Fudan University, 136 Yi Xue Yuan Road, 200032, Shanghai, China.
Qin Lun-Xiu
Ma Zeng-Chen
Ye Sheng-Long
Liu Yin-Kun
Ye Qing-Hai
Wu Xin
Huang Wei
Tang Zhao-You
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Article Info
Journal
Journal of cancer research and clinical oncology
Abbr.
J Cancer Res Clin Oncol
ISSN
0171-5216
DOI
10.1007/s00432-002-0407-5
Published
2003-05-00
Epub
2003-00-07
Pages
279-86
Language
English
Region
Germany
NLM ID
7902060
Subset
IM
External Links
PubMed source DOI: 10.1007/s00432-002-0407-5 View journal details
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