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PMID: 12734244 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two international methicillin-resistant Staphylococcus aureus clones endemic in a university hospital in Patras, Greece.

Journal of clinical microbiology ·Vol. 41 ·No. 5 ·2003-05-00 ·Pages 2027-32

Aires de Sousa M, Bartzavali C, Spiliopoulou I, Sanches IS, Crisóstomo MI, de Lencastre H

Abstract

Pulsed-field gel electrophoresis (PFGE) of SmaI macrofragments and hybridization of ClaI digests with the mecA- and Tn554-specific DNA probes were used to define the endemic clones of methicillin-resistant Staphylococcus aureus (MRSA) among strains collected in 1993 and 1998 to 2000 at the University Hospital of Patras, Patras, Greece. Representatives of each clonal type were analyzed by spaA typing, multilocus sequence typing (MLST), and staphylococcal chromosomal cassette mec (SCCmec) typing. The results indicated the existence of two successive international MRSA clones: (i) a clonal type with PFGE type A, sequence type (ST) 30 (ST30), and SCCmec type IV, which was very similar to a clone widely spread in the United Kingdom, Mexico, and Finland, and (ii) a clonal type with PFGE type B, ST239, and SCCmec III, which was related to the Brazilian clone. Both clones seem to be widespread in Greece as well. A novel MRSA clone is also described and is characterized by a new MLST type (ST80) associated with SCCmec type IV and with the presence of Panton-Valentine leukocidin genes.

MeSH Terms
Bacterial Typing Techniques Base Sequence Cross Infection/drug therapy,microbiology DNA, Bacterial/genetics Electrophoresis, Gel, Pulsed-Field Genes, Bacterial Genotype Greece Hospitals, University Humans Methicillin Resistance/genetics Phenotype Staphylococcal Infections/drug therapy,microbiology Staphylococcus aureus/classification,drug effects,genetics,isolation & purification
Chemicals
DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Aires de Sousa M
Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal.
Bartzavali C
Spiliopoulou I
Sanches I Santos
Crisóstomo M I
de Lencastre H
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.41.5.2027-2032.2003
Published
2003-05-00
Pages
2027-32
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC154747
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.41.5.2027-2032.2003 PMC Full Text View journal details
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