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PMID: 12730134 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.

Genes & development ·Vol. 17 ·No. 9 ·2003-05-01 ·Pages 1153-65

Takayama Y, Kamimura Y, Okawa M, Muramatsu S, Sugino A, Araki H

Abstract

Eukaryotic chromosomal DNA replication requires a two-step assembly of replication proteins on origins; formation of the prereplicative complex (pre-RC) in late M and G1 phases of the cell cycle, and assembly of other replication proteins in S phase to load DNA polymerases to initiate DNA synthesis. In budding yeast, assembly of Dpb11 and the Sld3-Cdc45 complex on the pre-RC at origins is required for loading DNA polymerases. Here we describe a novel replication complex, GINS (Go, Ichi, Nii, and San; five, one, two, and three in Japanese), in budding yeast, consisting of Sld5, Psf1 (partner of Sld five 1), Psf2, and Psf3 proteins, all of which are highly conserved in eukaryotic cells. Since the conditional mutations of Sld5 and Psf1 confer defect of DNA replication under nonpermissive conditions, GINS is suggested to function for chromosomal DNA replication. Consistently, in S phase, GINS associates first with replication origins and then with neighboring sequences. Without GINS, neither Dpb11 nor Cdc45 associates properly with chromatin DNA. Conversely, without Dpb11 or Sld3, GINS does not associate with origins. Moreover, genetic and two-hybrid interactions suggest that GINS interacts with Sld3 and Dpb11. Therefore, Dpb11, Sld3, Cdc45, and GINS assemble in a mutually dependent manner on replication origins to initiate DNA synthesis.

MeSH Terms
Amino Acid Sequence Cell Cycle Proteins/metabolism DNA Replication/physiology DNA, Fungal/genetics DNA-Binding Proteins/genetics,metabolism Fungal Proteins/metabolism Molecular Sequence Data Ribonucleoprotein, U4-U6 Small Nuclear Ribonucleoprotein, U5 Small Nuclear Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Alignment
Chemicals
Cell Cycle Proteins DNA, Fungal DNA-Binding Proteins DPB11 protein, S cerevisiae Fungal Proteins PRP8 protein, S cerevisiae Ribonucleoprotein, U4-U6 Small Nuclear Ribonucleoprotein, U5 Small Nuclear SLD5 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Takayama Yuko
Division of Microbial Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
Kamimura Yoichiro
Okawa Mariko
Muramatsu Sachiko
Sugino Akio
Araki Hiroyuki
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1065903
Published
2003-05-01
Pages
1153-65
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC196052
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1065903 PMC Full Text View journal details
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