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PMID: 12719596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The NB protein of influenza B virus is not necessary for virus replication in vitro.

Journal of virology ·Vol. 77 ·No. 10 ·2003-05-00 ·Pages 6050-4

Hatta M, Kawaoka Y

Abstract

The NB protein of influenza B virus is thought to function as an ion channel and therefore would be expected to have an essential function in viral replication. Because direct evidence for its absolute requirement in the viral life cycle is lacking, we generated NB knockout viruses by reverse genetics and tested their growth properties both in vitro and in vivo. Mutants not expressing NB replicated as efficiently as the wild-type virus in cell culture, whereas in mice they showed restricted growth compared with findings for the wild-type virus. Thus, the NB protein is not essential for influenza B virus replication in cell culture but promotes efficient growth in mice.

MeSH Terms
Animals Base Sequence Cell Line Dogs Female Humans Influenza B virus/genetics,metabolism,pathogenicity,physiology Influenza, Human/virology Ion Channels/chemistry,genetics,metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Mutation Viral Proteins/chemistry,genetics,metabolism Virus Replication
Chemicals
Ion Channels Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hatta Masato
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Kawaoka Yoshihiro
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.10.6050-6054.2003
Published
2003-05-00
Pages
6050-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC154028
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.10.6050-6054.2003 PMC Full Text View journal details
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