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PMID: 12554645 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.

The EMBO journal ·Vol. 22 ·No. 3 ·2003-02-03 ·Pages 438-49

Maekawa H, Usui T, Knop M, Schiebel E

Abstract

The budding yeast spindle aligns along the mother- bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1, mediates contacts of CMs with the cortex of the daughter cell, the bud. Here we established a novel series of events that target Kar9 to the bud cortex. First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9. Thirdly, Kar9 and Cdc28 then move from the SPB to the plus end of CMs directed towards the bud. This movement is dependent upon the microtubule motor protein Kip2. Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles.

MeSH Terms
CDC2 Protein Kinase/metabolism CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle/physiology Cell Cycle Proteins/metabolism Microscopy, Fluorescence Microtubule Proteins/metabolism Microtubules/metabolism Nuclear Proteins/metabolism Phosphorylation Protein Binding Protein Transport/physiology Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Spindle Apparatus/metabolism Two-Hybrid System Techniques
Chemicals
BIM1 protein, S cerevisiae Cell Cycle Proteins KAR9 protein, S cerevisiae Microtubule Proteins Nuclear Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins CDC2 Protein Kinase CDC28 Protein Kinase, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maekawa Hiromi
The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
Usui Takeo
Knop Michael
Schiebel Elmar
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg063
Published
2003-02-03
Pages
438-49
Language
English
Region
England
NLM ID
8208664
PMCID
PMC140747
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg063 PMC Full Text View journal details
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