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PMID: 12513974 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bacillus anthracis diverges from related clades of the Bacillus cereus group in 16S-23S ribosomal DNA intergenic transcribed spacers containing tRNA genes.

Applied and environmental microbiology ·Vol. 69 ·No. 1 ·2003-01-00 ·Pages 33-40

Cherif A, Borin S, Rizzi A, Ouzari H, Boudabous A, Daffonchio D

Abstract

Mung bean nuclease treatment of 16S-23S ribosomal DNA intergenic transcribed spacers (ITS) amplified from several strains of the six species of the Bacillus cereus group showed that B. anthracis Davis TE702 and B. mycoides G2 have other intermediate fragments in addition to the 220- and 550-bp homoduplex fragments typical of the B. cereus group. Long and intermediate homoduplex ITS fragments from strains Davis TE702 and G2 and from another 19 strains of the six species were sequenced. Two main types of ITS were found, either with two tRNA genes (tRNA(Ile) and tRNA(Ala)) or without any at all. Strain Davis TE702 harbors an additional ITS with a single tRNA gene, a hybrid between the tRNA(Ile) and tRNA(Ala) genes, suggesting that a recombination event rather than a deletion generated the single tDNA-containing ITS. Strain G2 showed an additional ITS of intermediate length with no tDNA and no similarity to other known sequences. Neighbor-joining analysis of tDNA-containing long ITS indicated that B. cereus and B. thuringiensis represent a single clade. Three signature sequences discriminated B. anthracis from B. cereus and B. thuringiensis, indicating that the anthrax agent started evolving separately from the related clades of the B. cereus group. B. mycoides and B. weienstephanensis were very closely related, while B. pseudomycoides appeared the most distant species.

MeSH Terms
Bacillus anthracis/classification,genetics Bacillus cereus/classification,genetics Base Sequence DNA, Ribosomal Spacer/analysis,genetics Genetic Variation Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics RNA, Transfer, Ala/genetics RNA, Transfer, Ile/genetics Sequence Analysis, DNA
Chemicals
DNA, Ribosomal Spacer RNA, Ribosomal, 16S RNA, Ribosomal, 23S RNA, Transfer, Ala RNA, Transfer, Ile
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cherif Ameur
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan, Italy.
Borin Sara
Rizzi Aurora
Ouzari Hadda
Boudabous Abdellatif
Daffonchio Daniele
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.1.33-40.2003
Published
2003-01-00
Pages
33-40
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC152393
Subset
IM
Databases
GENBANK
AJ420048, AJ420049, AJ420050, AJ420051, AJ420052, AJ420053, AJ420054, AJ420055, AJ420056, AJ420057, AJ420058, AJ420059, AJ420060, AJ420061, AJ420062, AJ420063, AJ420064, AJ420065, AJ420066, AJ420067, AJ420068, AJ420069, AJ420070, AJ420071
External Links
PubMed source DOI: 10.1128/AEM.69.1.33-40.2003 PMC Full Text View journal details
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