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PMID: 12462416 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expression.

Neurochemical research ·Vol. 27 ·No. 10 ·2002-10-00 ·Pages 1181-92

Freeman WM, Brebner K, Patel KM, Lynch WJ, Roberts DC, Vrana KE

Abstract

Repeated cocaine administration produces changes in gene expression that are thought to contribute to the behavioral alterations observed with cocaine abuse. This study focuses on gene expression changes in the frontal cortex, a component of reinforcement, sensory, associative, and executive circuitries. Changes in frontal cortex gene expression after repeated cocaine self-administration may lead to changes in the behaviors associated with this brain region. Rats self-administered cocaine for 10 days in a continuous access, discrete trial paradigm (averaging 100 mg/kg/day) and were examined for changes in relative frontal cortex mRNA abundance by cDNA hybridization arrays. Among the changes observed following array analysis, increased nerve-growth-factor-induced B (NGFI-B), adenylyl cyclase type VIII (AC VIII), and reduced cysteine-rich protein 2 (CRP2) mRNA were confirmed by quantitative RT-PCR. These changes share commonalities and exhibit differences with previous reports of gene expression changes in the frontal cortex after noncontingent cocaine administration.

MeSH Terms
Animals Cocaine/administration & dosage,pharmacology Frontal Lobe/drug effects,physiology Gene Expression/drug effects Immunoblotting Oligonucleotide Array Sequence Analysis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Self Administration
Chemicals
Cocaine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Freeman Willard M
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem. NC 27157-1083, USA. freemanw@ohsu.edu
Brebner Karen
Patel Kruti M
Lynch Wendy J
Roberts David C S
Vrana Kent E
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Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
0364-3190
DOI
10.1023/a:1020929526688
Email
freemanw@ohsu.edu
Published
2002-10-00
Pages
1181-92
Language
English
Region
United States
NLM ID
7613461
Subset
IM
Grants
NIDA NIH HHS · P50DA06643 · United States
NIDA NIH HHS · R01DA13770 · United States
NIDA NIH HHS · T32DA07246 · United States
External Links
PubMed source DOI: 10.1023/a:1020929526688 View journal details
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