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PMID: 12456830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties of mEPSCs recorded in layer II neurones of rat barrel cortex.

The Journal of physiology ·Vol. 545 ·No. 2 ·2002-00-01 ·Pages 509-20

Simkus CR, Stricker C

Abstract

Voltage-clamp recordings from layer II neurones in somatosensory cortex of rats aged between 12 and 17 days showed a high frequency of spontaneous postsynaptic currents (sPSCs), which on average was 33 +/- 13 Hz (s.d.). sPSCs were mediated largely by glutamatergic AMPA receptors. Their rates and amplitudes were independent of blocking sodium channels with 1 microM tetrodotoxin (TTX). Most of them, therefore, represent genuine miniature excitatory postsynaptic currents (mEPSCs). The rise time of the fastest (10 %) mEPSCs was 288 +/- 86 micros (10-90 %) and the half-width was 1073 +/- 532 micros. The amplitude was -5.9 +/- 1.1 pA with a coefficient of variation (CV) of 0.44 +/- 0.14. The rate of mEPSCs was very temperature sensitive with a Q(10) (33-37 degrees C) of 8.9 +/- 0.9. Due to this temperature sensitivity, we estimated that the microscope lamp contributed an increase in temperature of about 4 degrees C to the tissue in the focal volume of the condenser. Cell-type differences in the rate of mEPSCs were found between pyramidal/multipolar and bipolar cells. The latter had a frequency of about a third of that seen in the other cell groups. Recordings in layer II are ideally suited to investigate mechanisms of spontaneous transmitter release.

MeSH Terms
Action Potentials/drug effects,physiology Algorithms Animals Electrophysiology Excitatory Postsynaptic Potentials/drug effects,physiology Hippocampus/cytology,drug effects,physiology In Vitro Techniques Kinetics Lysine/analogs & derivatives Male Membrane Potentials/drug effects,physiology Neocortex/cytology,drug effects,physiology Neurons/drug effects,physiology Neurotransmitter Agents/metabolism Patch-Clamp Techniques Pyramidal Cells/drug effects,physiology Rats Rats, Wistar Sodium Channel Blockers/pharmacology Somatosensory Cortex/cytology,drug effects,physiology Synapses/drug effects,physiology Temperature
Chemicals
Neurotransmitter Agents Sodium Channel Blockers biocytin Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simkus Christopher R L
Institute of Neuroinformatics, University of Zürich and Federal Institute of Technology (ETH), Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
Stricker Christian
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.2002.022095
Published
2002-00-01
Pages
509-20
Language
English
Region
England
NLM ID
0266262
PMCID
PMC2290708
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.2002.022095 PMC Full Text View journal details
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