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PMID: 12456659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B.

The EMBO journal ·Vol. 21 ·No. 23 ·2002-12-02 ·Pages 6527-38

Mosammaparast N, Ewart CS, Pemberton LF

Abstract

Import of core histones into the nucleus is a prerequisite for their deposition onto DNA and the assembly of chromatin. Here we demonstrate that nucleosome assembly protein 1 (Nap1p), a protein previously implicated in the deposition of histones H2A and H2B, is also involved in the transport of these two histones. We demonstrate that Nap1p can bind directly to Kap114p, the primary karyopherin/importin responsible for the nuclear import of H2A and H2B. Nap1p also serves as a bridge between Kap114p and the histone nuclear localization sequence (NLS). Nap1p acts cooperatively to increase the affinity of Kap114p for these NLSs. Nuclear accumulation of histone NLS-green fluorescent protein (GFP) reporters was decreased in deltanap1 cells. Furthermore, we demonstrate that Nap1p promotes the association of the H2A and H2B NLSs specifically with the karyopherin Kap114p. Localization studies demonstrate that Nap1p is a nucleocytoplasmic shuttling protein, and genetic experiments suggest that its shuttling is important for maintaining chromatin structure in vivo. We propose a model in which Nap1p links the nuclear transport of H2A and H2B to chromatin assembly.

MeSH Terms
Active Transport, Cell Nucleus Cell Cycle Proteins Cell Nucleus/metabolism Histones/metabolism In Vitro Techniques Karyopherins/metabolism Nuclear Proteins/metabolism Nucleosome Assembly Protein 1 Protein Binding Proteins/metabolism Retroelements/genetics Saccharomyces cerevisiae Proteins Yeasts/genetics,metabolism beta Karyopherins
Chemicals
Cell Cycle Proteins Histones KAP114 protein, S cerevisiae Karyopherins NAP1 protein, S cerevisiae Nuclear Proteins Nucleosome Assembly Protein 1 Proteins Retroelements Saccharomyces cerevisiae Proteins beta Karyopherins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mosammaparast Nima
Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.
Ewart Courtney S
Pemberton Lucy F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf647
Published
2002-12-02
Pages
6527-38
Language
English
Region
England
NLM ID
8208664
PMCID
PMC136951
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065385 · United States
NIGMS NIH HHS · R01 GM65385 · United States
External Links
PubMed source DOI: 10.1093/emboj/cdf647 PMC Full Text View journal details
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