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PMID: 12426388 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nutrient control of gene expression in Drosophila: microarray analysis of starvation and sugar-dependent response.

The EMBO journal ·Vol. 21 ·No. 22 ·2002-11-15 ·Pages 6162-73

Zinke I, Schütz CS, Katzenberger JD, Bauer M, Pankratz MJ

Abstract

We have identified genes regulated by starvation and sugar signals in Drosophila larvae using whole-genome microarrays. Based on expression profiles in the two nutrient conditions, they were organized into different categories that reflect distinct physiological pathways mediating sugar and fat metabolism, and cell growth. In the category of genes regulated in sugar-fed, but not in starved, animals, there is an upregulation of genes encoding key enzymes of the fat biosynthesis pathway and a downregulation of genes encoding lipases. The highest and earliest activated gene upon sugar ingestion is sugarbabe, a zinc finger protein that is induced in the gut and the fat body. Identification of potential targets using microarrays suggests that sugarbabe functions to repress genes involved in dietary fat breakdown and absorption. The current analysis provides a basis for studying the genetic mechanisms underlying nutrient signalling.

MeSH Terms
Animal Feed Animals Carbohydrates/pharmacology Dietary Carbohydrates/pharmacology Dietary Fats/pharmacology Drosophila Proteins/biosynthesis,genetics,isolation & purification,physiology Drosophila melanogaster/drug effects,genetics,growth & development,metabolism Enzyme Induction/drug effects Gene Expression Regulation/drug effects Genes, Insect Lipase/biosynthesis,genetics Malus Oligonucleotide Array Sequence Analysis Pupa Reverse Transcriptase Polymerase Chain Reaction Starvation/genetics,metabolism Transcription Factors/biosynthesis,genetics,isolation & purification Yeasts Zinc Fingers
Chemicals
Carbohydrates Dietary Carbohydrates Dietary Fats Drosophila Proteins Transcription Factors sug protein, Drosophila Lipase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zinke Ingo
Institut für Genetik, Forschungszentrum Karlsruhe, Postfach 3640, D-76021 Karlsruhe, Germany.
Schütz Christina S
Katzenberger Jörg D
Bauer Matthias
Pankratz Michael J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf600
Published
2002-11-15
Pages
6162-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC137192
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf600 PMC Full Text View journal details
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