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PMID: 12414728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intron status and 3'-end formation control cotranscriptional export of mRNA.

Genes & development ·Vol. 16 ·No. 21 ·2002-11-01 ·Pages 2761-6

Lei EP, Silver PA

Abstract

Messenger RNA export factors are recruited to genes in a transcription-dependent manner. To ascertain the mechanism of this process, we show that RNA polymerase II transcription is sufficient to recruit the Saccharomyces cerevisiae hnRNP protein Npl3 to a gene independent of RNA sequence. In contrast, the cotranscriptional recruitment of the RNA-binding protein Yra1 is dependent on pre-mRNA processing. Yra1 associates with introns of intron-containing genes in a splicing-dependent manner. Conversely, Yra1 recruitment to genes without introns is not dependent on splicing. Finally, 3'-end formation is required for Yra1 recruitment to genes regardless of intron status.

MeSH Terms
Heterogeneous-Nuclear Ribonucleoproteins/genetics Introns/genetics Models, Molecular Nuclear Proteins/genetics RNA 3' End Processing/genetics RNA Splicing RNA, Fungal/genetics RNA, Messenger/chemistry,genetics RNA-Binding Proteins/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics Transcription, Genetic
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins Nuclear Proteins RNA, Fungal RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins YRA1 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lei Elissa P
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Silver Pamela A
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1032902
Published
2002-11-01
Pages
2761-6
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC187473
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1032902 PMC Full Text View journal details
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