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PMID: 11459827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Messenger RNAs are recruited for nuclear export during transcription.

Genes & development ·Vol. 15 ·No. 14 ·2001-07-15 ·Pages 1771-82

Lei EP, Krebber H, Silver PA

Abstract

Following transcription and processing, eukaryotic mRNAs are exported from the nucleus to the cytoplasm for translation. Here we present evidence that mRNAs are targeted for nuclear export cotranscriptionally. Combined mutations in the Saccharomyces cerevisiae hnRNP Npl3 and TATA-binding protein (TBP) block mRNA export, implying that cotranscriptional recruitment of Npl3 is required for efficient export of mRNA. Furthermore, Npl3 can be found in a complex with RNA Pol II, indicating that Npl3 associates with the transcription machinery. Finally, Npl3 is recruited to genes in a transcription dependent manner as determined by chromatin immunoprecipitation. Another mRNA export factor, Yra1, also associates with chromatin cotranscriptionally but appears to be recruited at a later step. Taken together, our results suggest that export factors are recruited to the sites of transcription to promote efficient mRNA export.

MeSH Terms
Biological Transport Cell Cycle Proteins/metabolism Cell Nucleus/metabolism Chromatin/metabolism DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Models, Genetic Mutation Nuclear Proteins/genetics,metabolism RNA Polymerase II/metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism RNA-Binding Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Cell Cycle Proteins Chromatin DNA-Binding Proteins Fungal Proteins NPL3 protein, S cerevisiae Nuclear Proteins RNA, Fungal RNA, Messenger RNA-Binding Proteins SPT15 protein, S cerevisiae Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Transcription Factors YRA1 protein, S cerevisiae RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lei E P
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Krebber H
Silver P A
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.892401
Published
2001-07-15
Pages
1771-82
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312744
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.892401 PMC Full Text View journal details
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