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PMID: 12368245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of a chromosomal rearrangement involved in the adaptive evolution of yeast strains.

Genome research ·Vol. 12 ·No. 10 ·2002-10-00 ·Pages 1533-9

Pérez-Ortín JE, Querol A, Puig S, Barrio E

Abstract

Wine yeast strains show a high level of chromosome length polymorphism. This polymorphism is mainly generated by illegitimate recombination mediated by Ty transposons or subtelomeric repeated sequences. We have found, however, that the SSU1-R allele, which confers sulfite resistance to yeast cells, is the product of a reciprocal translocation between chromosomes VIII and XVI due to unequal crossing-over mediated by microhomology between very short sequences on the 5' upstream regions of the SSU1 and ECM34 genes. We also show that this translocation is only present in wine yeast strains, suggesting that the use for millennia of sulfite as a preservative in wine production could have favored its selection. This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae.

MeSH Terms
Anion Transport Proteins/genetics Base Sequence/genetics Chromosomes, Fungal/genetics DNA, Fungal/genetics Evolution, Molecular Gene Frequency/genetics Gene Rearrangement/genetics Genes, Fungal/genetics Genetic Markers/genetics Genome, Fungal Molecular Sequence Data Recombination, Genetic/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics Species Specificity Translocation, Genetic/genetics
Chemicals
Anion Transport Proteins DNA, Fungal Genetic Markers Saccharomyces cerevisiae Proteins Ssu1 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pérez-Ortín José E
Departament de Bioquímica i Biologia Molecular, Universitat de València, Spain. jose.e.perez@uv.es
Querol Amparo
Puig Sergi
Barrio Eladio
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.436602
Email
jose.e.perez@uv.es
Published
2002-10-00
Pages
1533-9
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC187534
Subset
IM
Databases
GENBANK
AF239757, AF239758, AJ458340, AJ458341, AJ458342, AJ458343, AJ458344, AJ458345, AJ458346, AJ458347, AJ458348, AJ458349, AJ458350, AJ458351, AJ458352, AJ458353, AJ458354, AJ458355, AJ458356, AJ458357, AJ458358, AJ458359, AJ458360, AJ458361, AJ458362, AJ458363, AJ458364, AJ458365, AJ458366, AJ458367
External Links
PubMed source DOI: 10.1101/gr.436602 PMC Full Text View journal details
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