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PMID: 12356865 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone.

The Journal of cell biology ·Vol. 158 ·No. 7 ·2002-09-30 ·Pages 1207-17

Diefenbach TJ, Latham VM, Yimlamai D, Liu CA, Herman IM, Jay DG

Abstract

The myosin family of motor proteins is implicated in mediating actin-based growth cone motility, but the roles of many myosins remain unclear. We previously implicated myosin 1c (M1c; formerly myosin I beta) in the retention of lamellipodia (Wang et al., 1996). Here we address the role of myosin II (MII) in chick dorsal root ganglion neuronal growth cone motility and the contribution of M1c and MII to retrograde F-actin flow using chromophore-assisted laser inactivation (CALI). CALI of MII reduced neurite outgrowth and growth cone area by 25%, suggesting a role for MII in lamellipodial expansion. Micro-CALI of MII caused a rapid reduction in local lamellipodial protrusion in growth cones with no effects on filopodial dynamics. This is opposite to micro-CALI of M1c, which caused an increase in lamellipodial protrusion. We used fiduciary beads (Forscher et al., 1992) to observe retrograde F-actin flow during the acute loss of M1c or MII. Micro-CALI of M1c reduced retrograde bead flow by 76%, whereas micro-CALI of MII or the MIIB isoform did not. Thus, M1c and MIIB serve opposite and nonredundant roles in regulating lamellipodial dynamics, and M1c activity is specifically required for retrograde F-actin flow.

MeSH Terms
Actins/chemistry Animals Antibody Formation Antibody Specificity Cell Movement/physiology Chick Embryo Ganglia, Spinal/embryology,metabolism Growth Cones/physiology Lasers Myosin Type I/immunology,metabolism Neurites/metabolism Neurons/metabolism Nonmuscle Myosin Type IIB/immunology,metabolism Peptide Fragments/immunology Rabbits
Chemicals
Actins Peptide Fragments Myosin Type I Nonmuscle Myosin Type IIB
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Diefenbach Thomas J
Department of Physiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Latham Vaughan M
Yimlamai Dean
Liu Canwen A
Herman Ira M
Jay Daniel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200202028
Published
2002-09-30
Pages
1207-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173244
Subset
IM
Grants
NEI NIH HHS · R01 EY011992 · United States
NIDDK NIH HHS · DKSP3034928 · United States
NINDS NIH HHS · NS34699 · United States
NEI NIH HHS · EY90933 · United States
NIDDK NIH HHS · DK07542 · United States
NIGMS NIH HHS · GM55110 · United States
NINDS NIH HHS · R01 NS034699 · United States
NIDDK NIH HHS · T32 DK007542 · United States
NEI NIH HHS · EY11992 · United States
External Links
PubMed source DOI: 10.1083/jcb.200202028 PMC Full Text View journal details
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