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PMID: 12235208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

IkappaB kinase signaling is essential for maintenance of mature B cells.

The Journal of experimental medicine ·Vol. 196 ·No. 6 ·2002-09-16 ·Pages 743-52

Pasparakis M, Schmidt-Supprian M, Rajewsky K

Abstract

Nuclear factor (NF)-kappaB proteins play crucial roles in immune responses and cellular survival. Activation of NF-kappaB is mediated by the IkappaB kinase (IKK) complex, which is composed of two kinases, IKK1 and IKK2, and a regulatory subunit termed NF-kappaB essential modulator (NEMO). IKK2- and NEMO-deficient mice die at early embryonic stages. We therefore used conditional gene targeting to evaluate the role of these proteins in B cells in adult mice. B lineage-specific disruption of either IKK signaling by deletion of NEMO, or of IKK2-specific signals by ablation of IKK2 activity leads to the disappearance of mature B lymphocytes. We conclude that maintenance of mature B cells depends on IKK-mediated activation of NF-kappaB.

MeSH Terms
Animals Antigens, CD19/physiology B-Lymphocytes/physiology Flow Cytometry I-kappa B Kinase Integrases/physiology Mice NF-kappa B/physiology Protein Serine-Threonine Kinases/physiology Viral Proteins/physiology
Chemicals
Antigens, CD19 NF-kappa B Viral Proteins Protein Serine-Threonine Kinases Chuk protein, mouse I-kappa B Kinase Ikbkb protein, mouse Ikbke protein, mouse Cre recombinase Integrases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pasparakis Manolis
Institute for Genetics, University of Cologne, D-50931 Cologne, Germany.
Schmidt-Supprian Marc
Rajewsky Klaus
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20020907
Published
2002-09-16
Pages
743-52
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2194059
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.20020907 PMC Full Text View journal details
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