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PMID: 12228594 Published · ppublish English Journal Article

Changes in Protein Isoprenylation during the Growth of Suspension-Cultured Tobacco Cells.

Plant physiology ·Vol. 109 ·No. 1 ·1995-09-00 ·Pages 277-284

Morehead TA, Biermann BJ, Crowell DN, Randall SK

Abstract

Isoprenylation facilitates the association of proteins with intracellular membranes and/or other proteins. In mammalian and yeast cells, isoprenylated proteins are involved in signal transduction, cell division, organization of the cytoskeleton, and vesicular transport. Recently, protein isoprenylation has been demonstrated in higher plants, but little is currently known about the functions of isoprenylated plant proteins. We report that inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase (lovastatin) or prenyl:protein transferases (perilly alcohol) severely impair the growth of cultured tobacco (Nicotiana tabacum) cells but only when added within the first 2 d following transfer to fresh medium, before any increase in culture volume is detectable. This "window" of sensitivity to inhibitors of protein isoprenylation correlates temporally with an increase in [14C]mevalonate incorporation into tobacco cell proteins in vitro. We have also observed a marked increase in farnesyl:protein transferase activity at this early time in the growth of tobacco cultures. In contrast, type I geranylgeranyl:protein transferase activity does not change significantly during culture growth. Although these events coincide with the replication of DNA, I [mu]M lovastatin-treated cells are capable of DNA synthesis, suggesting that lovastatin-induced cell growth arrest is not due to inhibition of DNA replication. Together, these data support the hypothesis that protein isoprenylation is necessary for the early stages of growth of tobacco cultures.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morehead T. A.
Biology Department, Indiana University-Purdue University at Indianapolis, 723 West Michigan Street, Indianapolis, Indiana 46202-5132.
Biermann B. J.
Crowell D. N.
Randall S. K.
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.109.1.277
Published
1995-09-00
Pages
277-284
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC157586
External Links
PubMed source DOI: 10.1104/pp.109.1.277 PMC Full Text View journal details
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