Identification of geranylgeranyl-modified proteins in HeLa cells.

Science (New York, N.Y.) ·Vol. 247 ·No. 4940 ·1990-01-19 ·Pages 320-2

Farnsworth CC, Gelb MH, Glomset JA

Abstract

Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.

MeSH Terms

Chromatography, Gel Chromatography, Ion Exchange Diterpenes/metabolism Gas Chromatography-Mass Spectrometry HeLa Cells/metabolism Humans Mevalonic Acid/metabolism Molecular Structure Nickel Peptide Fragments Proteins/metabolism

Chemicals

Diterpenes Peptide Fragments Proteins Nickel Mevalonic Acid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Farnsworth C C

Howard Hughes Medical Institute Laboratory, University of Washington, Seattle 98195.

Gelb M H

Glomset J A

References (13)

13 references, click to expand

Isoprenoid synthesis during the cell cycle. Studies of 3-hydroxy-3-methylglutaryl-coenzyme A synthase and reductase and isoprenoid labeling in cells synchronized by centrifugal elutriation.
J Biol Chem. 1988 Jul 25;263(21):10104-10 PMID: 2899074
Evidence for modification of lamin B by a product of mevalonic acid.
J Biol Chem. 1988 May 5;263(13):5997-6000 PMID: 3283116
Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.
J Biol Chem. 1988 Dec 5;263(34):18236-40 PMID: 3056940
Cellular distribution of cholesterogenesis-linked, phosphoisoprenylated proteins in proliferating cells.
FEBS Lett. 1989 Mar 13;245(1-2):110-6 PMID: 2924914
All ras proteins are polyisoprenylated but only some are palmitoylated.
Cell. 1989 Jun 30;57(7):1167-77 PMID: 2661017
Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans.
Science. 1989 Jul 28;245(4916):379-85 PMID: 2569235
Prenylated proteins from kidney.
Biochem Biophys Res Commun. 1986 Aug 29;139(1):209-14 PMID: 3094509
Defective macromolecule biosynthesis and cell-cycle progression in a mammalian cell starved for mevalonate.
Proc Natl Acad Sci U S A. 1985 May;82(10):3257-61 PMID: 2582409
Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins.
J Biol Chem. 1984 Aug 25;259(16):10175-80 PMID: 6565705
Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases.
Proc Natl Acad Sci U S A. 1988 Jul;85(13):4643-7 PMID: 3290900
Human lamin B contains a farnesylated cysteine residue.
J Biol Chem. 1989 Dec 5;264(34):20422-9 PMID: 2684976
Isoprenylated proteins in cultured cells: subcellular distribution and changes related to altered morphology and growth arrest induced by mevalonate deprivation.
J Cell Physiol. 1987 Dec;133(3):471-81 PMID: 3693410
Incorporation of a product of mevalonic acid metabolism into proteins of Chinese hamster ovary cell nuclei.
J Cell Biol. 1988 Oct;107(4):1307-16 PMID: 3170631

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.2296721

Published

1990-01-19

Pages

320-2

Language

English

Region

United States

NLM ID

0404511

PMCID

PMC3442113

Subset

Grants

NHLBI NIH HHS · R01 HL036235 · United States

External Links

PubMed source DOI: 10.1126/science.2296721 PMC Full Text View journal details

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Identification of geranylgeranyl-modified proteins in HeLa cells.

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