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PMID: 12228259 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The alternative sigma factor sigma(E) plays an important role in intestinal survival and virulence in Vibrio cholerae.

Infection and immunity ·Vol. 70 ·No. 10 ·2002-10-00 ·Pages 5355-62

Kovacikova G, Skorupski K

Abstract

The alternative sigma factor sigma(E) (RpoE) is involved in the response to extracytoplasmic stress and plays a role in the virulence of a variety of different bacteria. To assess the role of sigma(E) in Vibrio cholerae pathogenesis, a DeltarpoE mutant was constructed and analyzed using the infant mouse model. The results here show that sigma(E) contributes significantly to the virulence of V. cholerae. The DeltarpoE mutant was highly attenuated with a 50% lethal dose more than 3 logs higher than that for the parental strain, and its ability to colonize the intestine was reduced approximately 30-fold. A time course of infection revealed that the number of CFU of the DeltarpoE mutant was approximately 1 log lower than that of the parental strain by 12 h postinoculation and decreased further by 24 h. The defect in virulence in the DeltarpoE mutant thus appears to be a diminished ability to survive within the intestinal environment. The results here also show that sigma(E) is not required for growth and survival of V. cholerae in vitro at high temperatures but is required under other stressful conditions, such as in the presence of 3% ethanol. As in Escherichia coli, the expression of rpoE in V. cholerae is dependent upon two promoters located upstream of the gene, P1 and P2. P1 appears to be sigma(70) dependent, whereas the downstream promoter, P2, is positively autoregulated by sigma(E).

MeSH Terms
Animals Animals, Suckling Bacterial Proteins/genetics,physiology Base Sequence Cholera/microbiology DNA, Bacterial/genetics Gene Deletion Gene Expression Regulation, Bacterial Genes, Bacterial Intestines/microbiology Mice Mutation Promoter Regions, Genetic Sigma Factor/genetics,physiology Transcription Factors/genetics,physiology Vibrio cholerae/genetics,growth & development,pathogenicity,physiology Virulence/genetics,physiology
Chemicals
Bacterial Proteins DNA, Bacterial Sigma Factor Transcription Factors sporulation-specific sigma factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kovacikova Gabriela
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Skorupski Karen
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.70.10.5355-5362.2002
Published
2002-10-00
Pages
5355-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC128310
Subset
IM
Grants
NIAID NIH HHS · R01 AI041558 · United States
NIAID NIH HHS · R29 AI041558 · United States
NIAID NIH HHS · AI-41558 · United States
External Links
PubMed source DOI: 10.1128/IAI.70.10.5355-5362.2002 PMC Full Text View journal details
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