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PMID: 12206310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus.

Virus genes ·Vol. 25 ·No. 1 ·2002-00-00 ·Pages 67-73

Matsuura Y, Tohya Y, Nakamura K, Shimojima M, Roerink F, Mochizuki M, Takase K, Akashi H, Sugimura T

Abstract

The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12-5801; ORF2, nt 5805-7880; and ORF3, nt 7877-8278). ORF1 encoded a polyprotein (calculated Mr of 214,802) which had the conserved motifs of non-structural proteins of other caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus. Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus.

MeSH Terms
Animals Base Sequence Blotting, Northern Caliciviridae/classification,genetics Conserved Sequence DNA, Viral/analysis Dogs Genome, Viral Molecular Sequence Data Open Reading Frames Phylogeny Poly A/chemistry Viral Nonstructural Proteins/genetics
Chemicals
DNA, Viral Viral Nonstructural Proteins Poly A
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Matsuura Yuichi
Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Japan.
Tohya Yukinobu
Nakamura Kazuya
Shimojima Masayuki
Roerink Frank
Mochizuki Masami
Takase Kozo
Akashi Hiroomi
Sugimura Takaaki
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1023/a:1020174225622
Published
2002-00-00
Pages
67-73
Language
English
Region
United States
NLM ID
8803967
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1020174225622 View journal details
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