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PMID: 12202376 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical and photophysical characterization of a BODIPY phosphatidylcholine as a membrane probe.

Biophysical journal ·Vol. 83 ·No. 3 ·2002-09-00 ·Pages 1511-24

Dahim M, Mizuno NK, Li XM, Momsen WE, Momsen MM, Brockman HL

Abstract

Lipids containing the dimethyl BODIPY fluorophore are used in cell biology because their fluorescence properties change with fluorophore concentration (C.-S. Chen, O. C. Martin, and R. E. Pagano. 1997. Biophys J. 72:37-50). The miscibility and steady-state fluorescence behavior of one such lipid, 1-palmitoyl-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-sn-glycero-3-phosphocholine (PBPC), have been characterized in mixtures with 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC). PBPC packs similarly to phosphatidylcholines having a cis-unsaturated acyl chain and mixes nearly ideally with SOPC, apparently without fluorophore-fluorophore aggregation. Increasing PBPC mole fraction from 0.0 to 1.0 in SOPC membranes changes the emission characteristics of the probe in a continuous manner. Analysis of these changes shows that emission from the excited dimethyl BODIPY monomer self quenches with a critical radius of 25.9 A. Fluorophores sufficiently close (< or =13.7 A) at the time of excitation can form an excited dimer, emission from which depends strongly on total lipid packing density. Overall, the data show that PBPC is a reasonable physical substitute for other phosphatidylcholines in fluid membranes. Knowledge of PBPC fluorescence in lipid monolayers has been exploited to determine the two-dimensional concentration of SOPC in unilamellar, bilayer membranes.

MeSH Terms

Biophysical Phenomena Biophysics Boron Compounds/chemistry Cell Membrane/metabolism Lipid Bilayers/chemistry Models, Theoretical Phosphatidylcholines/chemistry Pressure Spectrometry, Fluorescence Temperature

Chemicals

1-palmitoyl-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-sn-glycero-3-phosphocholine 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene Boron Compounds Lipid Bilayers Phosphatidylcholines

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Dahim Mohammed

The Hormel Institute, University of Minnesota, Austin, Minnesota 55912 USA.

Mizuno Nancy K

Li Xin-Min

Momsen William E

Momsen Maureen M

Brockman Howard L

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(02)73921-0

Published

2002-09-00

Pages

1511-24

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1302249

Subset

Grants

NHLBI NIH HHS · HL49180 · United States

External Links

PubMed source DOI: 10.1016/S0006-3495(02)73921-0 PMC Full Text View journal details

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Physical and photophysical characterization of a BODIPY phosphatidylcholine as a membrane probe.

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