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PMID: 9449334 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Acyl chain-length asymmetry alters the interfacial elastic interactions of phosphatidylcholines.

Biophysical journal ·Vol. 74 ·No. 1 ·1998-01-00 ·Pages 338-48

Ali S, Smaby JM, Momsen MM, Brockman HL, Brown RE

Abstract

Phosphatidylcholines (PCs) with stearoyl (18:0) sn-1 chains and variable-length, saturated sn-2 acyl chains were synthesized and investigated using a Langmuir-type film balance. Surface pressure was monitored as a function of lipid molecular area at various constant temperatures between 10 degrees C and 30 degrees C. Over this temperature range, 18:0-10:0 PC displayed only liquid-expanded behavior. In contrast, di-14:0 PC displayed liquid-expanded behavior at 24 degrees C and 30 degrees C, but two-dimensional phase transitions were evident at 20 degrees C, 15 degrees C, and 10 degrees C. The average molecular area of 18:0-10:0 PC was larger than that of liquid-expanded di-14:0 PC at equivalent surface pressures, and the shapes of their liquid expanded isotherms were somewhat dissimilar. Analysis of the elastic moduli of area compressibility (Cs(-1)) as a function of molecular area revealed shallower slopes in the semilog plots of 18:0-10:0 PC compared to di-14:0 PC. At membrane-like surface pressures (e.g., 30 mN/m), 18:0-10:0 PC was 20-25% more elastic (in an in-plane sense) than di-14:0 PC. Other PCs with varying degrees of chain-length asymmetry (18:0-8:0 PC, 18:0-12:0 PC, 18:0-14:0 PC, 18:0-16:0 PC) were also investigated to determine whether the higher in-plane elasticity of fluid-phase 18:0-10:0 PC is a common feature of PCs with asymmetrical chain lengths. Two-dimensional phase transitions in 18:0-14:0 PC and 18:0-16:0 PC prevented meaningful comparison with other fluid-phase PCs at 30 mN/m. However, the Cs(-1) values for fluid-phase 18:0-8:0 PC and 18:0-12:0 PC were similar to that of 18:0-10:0 PC (85-90 mN/m). These values showed chain-length asymmetrical PCs to have 20-25% greater in-plane elasticity than fluid-phase PCs with mono- or diunsaturated acyl chains.

MeSH Terms
Elasticity Phosphatidylcholines/chemical synthesis,chemistry Pressure Stearic Acids Structure-Activity Relationship Surface Properties Thermodynamics
Chemicals
Phosphatidylcholines Stearic Acids stearic acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ali S
The Hormel Institute, University of Minnesota, Austin 55912, USA. sali@lipo.com
Smaby J M
Momsen M M
Brockman H L
Brown R E
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(98)77791-4
Email
sali@lipo.com
Published
1998-01-00
Pages
338-48
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1299386
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045928 · United States
NIGMS NIH HHS · GM45928 · United States
NHLBI NIH HHS · HL17371 · United States
NHLBI NIH HHS · HL49180 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(98)77791-4 PMC Full Text View journal details
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