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PMID: 12123457 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Vibrio cholerae CytR is a repressor of biofilm development.

Molecular microbiology ·Vol. 45 ·No. 2 ·2002-07-00 ·Pages 471-83

Haugo AJ, Watnick PI

Abstract

Vibrio cholerae is both a human pathogen and a natural inhabitant of aquatic environments. In the aquatic environment, microorganisms are found attached to surfaces in structures known as biofilms. We have identified a transcriptional repressor in V. cholerae that inhibits exopolysaccharide synthesis and biofilm development. Our studies show that this repressor is the V. cholerae homologue of Escherichia coli CytR, a protein that represses nucleoside uptake and catabolism when nucleosides are scarce. We propose that the role of CytR in V. cholerae biofilm development is to co-ordinate bacterial biofilm accumulation with the presence of nucleosides. Thus, nucleosides may be a signal to planktonic cells to join the biofilm.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,physiology Biofilms/growth & development Cytidine/pharmacology Escherichia coli/physiology Escherichia coli Proteins/biosynthesis,genetics,physiology Gene Expression Regulation, Bacterial/drug effects,physiology Genes, Reporter Genetic Complementation Test Molecular Sequence Data Nucleosides/metabolism Phenotype Polysaccharides, Bacterial/biosynthesis Recombinant Fusion Proteins/physiology Repressor Proteins/genetics,physiology Sequence Alignment Sequence Deletion Sequence Homology, Amino Acid Species Specificity Transcription, Genetic Vibrio cholerae/genetics,physiology
Chemicals
Bacterial Proteins CytR protein, Vibrio cholerae Escherichia coli Proteins Nucleosides Polysaccharides, Bacterial Recombinant Fusion Proteins Repressor Proteins udp protein, E coli Cytidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haugo Adam J
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, 750 Washington St., Box 041, Boston, MA 02111, USA.
Watnick Paula I
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Article Info
Journal
Molecular microbiology
Abbr.
Mol Microbiol
ISSN
0950-382X
DOI
10.1046/j.1365-2958.2002.03023.x
Published
2002-07-00
Pages
471-83
Language
English
Region
England
NLM ID
8712028
PMCID
PMC2515492
Subset
IM
Grants
NIDDK NIH HHS · P30 DK34928 · United States
NIDDK NIH HHS · P30 DK034928 · United States
NIAID NIH HHS · R01 AI050032 · United States
NIAID NIH HHS · R01 AI050032-02 · United States
NIAID NIH HHS · K08 AI01588 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2958.2002.03023.x PMC Full Text View journal details
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