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PMID: 10572149 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Negative control of flagellum synthesis in Pseudomonas aeruginosa is modulated by the alternative sigma factor AlgT (AlgU).

Journal of bacteriology ·Vol. 181 ·No. 23 ·1999-12-00 ·Pages 7401-4

Garrett ES, Perlegas D, Wozniak DJ

Abstract

Many respiratory isolates of Pseudomonas aeruginosa from cystic fibrosis patients are mucoid (alginate producing) yet lack flagella. It was hypothesized that an alginate regulator inhibits flagellar gene expression. Mutations in algB, algR, and algT resulted in nonmucoid derivatives, yet algT mutants expressed flagella. AlgT-dependent control of flagellum synthesis occurred through inhibition of fliC but not rpoN transcription.

MeSH Terms
Alginates/metabolism Bacterial Proteins/genetics,physiology Carbohydrate Dehydrogenases/genetics Catechol 2,3-Dioxygenase DNA-Binding Proteins Dioxygenases Flagella/genetics,metabolism Flagellin/genetics Gene Expression Regulation, Bacterial Microscopy, Electron Movement Mutation Oxygenases/genetics Phenotype Pseudomonas aeruginosa/genetics,metabolism,physiology Sigma Factor/metabolism Trans-Activators Transcription Factors/genetics Transcription, Genetic
Chemicals
AlgR protein, Pseudomonas aeruginosa AlgU protein, Pseudomonas aeruginosa Alginates Bacterial Proteins DNA-Binding Proteins Sigma Factor Trans-Activators Transcription Factors Flagellin AlgB protein, Pseudomonas Carbohydrate Dehydrogenases GDPmannose dehydrogenase Oxygenases Dioxygenases Catechol 2,3-Dioxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Garrett E S
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.
Perlegas D
Wozniak D J
References (22)
22 references, click to expand
  1. Flagellar preparations from Pseudomonas aeruginosa: isolation and characterization.
    Infect Immun. 1982 Jan;35(1):281-8 PMID: 6797949
  2. Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.
    J Bacteriol. 1999 Jan;181(1):107-16 PMID: 9864319
  3. Flagella and motility alterations in Pseudomonas aeruginosa strains from patients with cystic fibrosis: relationship to patient clinical condition.
    Infect Immun. 1985 Nov;50(2):577-82 PMID: 3932214
  4. Cloning of genes from mucoid Pseudomonas aeruginosa which control spontaneous conversion to the alginate production phenotype.
    J Bacteriol. 1988 Apr;170(4):1452-60 PMID: 2965141
  5. Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):1954-7 PMID: 2564676
  6. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene.
    J Bacteriol. 1990 Jan;172(1):389-96 PMID: 2152909
  7. The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis.
    Mol Microbiol. 1992 Feb;6(4):459-69 PMID: 1560774
  8. Amino-terminal amino acids modulate sigma-factor DNA-binding activity.
    Genes Dev. 1993 Dec;7(12A):2446-55 PMID: 8253389
  9. Nonmotility and phagocytic resistance of Pseudomonas aeruginosa isolates from chronically colonized patients with cystic fibrosis.
    Infect Immun. 1994 Feb;62(2):596-605 PMID: 8300217
  10. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB, and algD reveals a hierarchy of alginate gene expression which is modulated by algT.
    J Bacteriol. 1994 Oct;176(19):6007-14 PMID: 7928961
  11. Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation.
    J Bacteriol. 1994 Nov;176(21):6677-87 PMID: 7961421
  12. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa.
    Gene. 1995 May 26;158(1):15-22 PMID: 7789804
  13. Cloning and phenotypic characterization of fleS and fleR, new response regulators of Pseudomonas aeruginosa which regulate motility and adhesion to mucin.
    Infect Immun. 1995 Dec;63(12):4868-76 PMID: 7591148
  14. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia.
    Microbiol Rev. 1996 Sep;60(3):539-74 PMID: 8840786
  15. Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription.
    Mol Microbiol. 1996 Oct;22(1):97-108 PMID: 8899712
  16. A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner.
    J Bacteriol. 1997 Sep;179(17):5574-81 PMID: 9287015
  17. Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection.
    Infect Immun. 1998 Jan;66(1):43-51 PMID: 9423837
  18. Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa.
    J Bacteriol. 1998 Feb;180(4):956-68 PMID: 9473053
  19. The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion.
    Infect Immun. 1998 Mar;66(3):1000-7 PMID: 9488388
  20. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.
    Gene. 1998 May 28;212(1):77-86 PMID: 9661666
  21. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development.
    Mol Microbiol. 1998 Oct;30(2):295-304 PMID: 9791175
  22. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.23.7401-7404.1999
Published
1999-12-00
Pages
7401-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC103708
Subset
IM
Grants
NCI NIH HHS · P30 CA012197 · United States
NHLBI NIH HHS · R01 HL058334 · United States
NHLBI NIH HHS · 5R01 HL58334 · United States
NCI NIH HHS · CA-12197 · United States
External Links
PubMed source DOI: 10.1128/JB.181.23.7401-7404.1999 PMC Full Text View journal details
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